Differential tyrosine kinase C mRNA distribution in extensor digitorum longus and soleus motoneurons in adult rats: Effect of axotomy and neurotrophin-3 treatment

Magda Simon; Dawn Mann; Gary Coulton; Giorgio Terenghi

doi:10.1016/S0304-3940(02)00007-1

Differential tyrosine kinase C mRNA distribution in extensor digitorum longus and soleus motoneurons in adult rats: Effect of axotomy and neurotrophin-3 treatment

Magda Simon, Dawn Mann, Gary Coulton, Giorgio Terenghi

Research output: Contribution to journal › Article › peer-review

Abstract

Delivery of neurotrophin-3 (NT-3) to severed sciatic nerves results in specific normalization of atrophied fast 2b gastrocnemius muscle fibres, and promotes preferential neuromuscular junction maturation of fast extensor digitorum longus (EDL). To investigate the selective influence on fast muscle reinnervation due to NT-3 delivery, we analyzed tyrosine kinase C (trkC) mRNA differential expression in EDL and slow soleus motor pools of unoperated rats and at 1-week post-axotomy. Motoneurons (Mns) were identified using retrograde tracers. TrkC mRNA quantification was estimated by silver grain counting. TrkC mRNA expression was higher in EDL than in soleus Mns in unoperated rats. Axotomy decreased trkC mRNA expression only in EDL Mns, this downregulation being prevented by NT-3. These results suggest that differential expression of trkC receptor is the morphological correlate of the preferential effect of NT-3 upon EDL Mns. © 2002 Elsevier Science Ireland Ltd. All rights reserved.

Original language	English
Pages (from-to)	9-12
Number of pages	3
Journal	Neuroscience letters
Volume	320
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3940(02)00007-1
Publication status	Published - 1 Mar 2002

Keywords

Fast motor unit
Nerve injury
Neurotrophin
Neurotrophin-3
Slow motor unit
Tyrosine kinase C

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10.1016/S0304-3940(02)00007-1

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title = "Differential tyrosine kinase C mRNA distribution in extensor digitorum longus and soleus motoneurons in adult rats: Effect of axotomy and neurotrophin-3 treatment",

abstract = "Delivery of neurotrophin-3 (NT-3) to severed sciatic nerves results in specific normalization of atrophied fast 2b gastrocnemius muscle fibres, and promotes preferential neuromuscular junction maturation of fast extensor digitorum longus (EDL). To investigate the selective influence on fast muscle reinnervation due to NT-3 delivery, we analyzed tyrosine kinase C (trkC) mRNA differential expression in EDL and slow soleus motor pools of unoperated rats and at 1-week post-axotomy. Motoneurons (Mns) were identified using retrograde tracers. TrkC mRNA quantification was estimated by silver grain counting. TrkC mRNA expression was higher in EDL than in soleus Mns in unoperated rats. Axotomy decreased trkC mRNA expression only in EDL Mns, this downregulation being prevented by NT-3. These results suggest that differential expression of trkC receptor is the morphological correlate of the preferential effect of NT-3 upon EDL Mns. {\textcopyright} 2002 Elsevier Science Ireland Ltd. All rights reserved.",

keywords = "Fast motor unit, Nerve injury, Neurotrophin, Neurotrophin-3, Slow motor unit, Tyrosine kinase C",

author = "Magda Simon and Dawn Mann and Gary Coulton and Giorgio Terenghi",

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T1 - Differential tyrosine kinase C mRNA distribution in extensor digitorum longus and soleus motoneurons in adult rats: Effect of axotomy and neurotrophin-3 treatment

AU - Simon, Magda

AU - Mann, Dawn

AU - Coulton, Gary

AU - Terenghi, Giorgio

N1 - 0304-3940Journal Article

PY - 2002/3/1

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N2 - Delivery of neurotrophin-3 (NT-3) to severed sciatic nerves results in specific normalization of atrophied fast 2b gastrocnemius muscle fibres, and promotes preferential neuromuscular junction maturation of fast extensor digitorum longus (EDL). To investigate the selective influence on fast muscle reinnervation due to NT-3 delivery, we analyzed tyrosine kinase C (trkC) mRNA differential expression in EDL and slow soleus motor pools of unoperated rats and at 1-week post-axotomy. Motoneurons (Mns) were identified using retrograde tracers. TrkC mRNA quantification was estimated by silver grain counting. TrkC mRNA expression was higher in EDL than in soleus Mns in unoperated rats. Axotomy decreased trkC mRNA expression only in EDL Mns, this downregulation being prevented by NT-3. These results suggest that differential expression of trkC receptor is the morphological correlate of the preferential effect of NT-3 upon EDL Mns. © 2002 Elsevier Science Ireland Ltd. All rights reserved.

AB - Delivery of neurotrophin-3 (NT-3) to severed sciatic nerves results in specific normalization of atrophied fast 2b gastrocnemius muscle fibres, and promotes preferential neuromuscular junction maturation of fast extensor digitorum longus (EDL). To investigate the selective influence on fast muscle reinnervation due to NT-3 delivery, we analyzed tyrosine kinase C (trkC) mRNA differential expression in EDL and slow soleus motor pools of unoperated rats and at 1-week post-axotomy. Motoneurons (Mns) were identified using retrograde tracers. TrkC mRNA quantification was estimated by silver grain counting. TrkC mRNA expression was higher in EDL than in soleus Mns in unoperated rats. Axotomy decreased trkC mRNA expression only in EDL Mns, this downregulation being prevented by NT-3. These results suggest that differential expression of trkC receptor is the morphological correlate of the preferential effect of NT-3 upon EDL Mns. © 2002 Elsevier Science Ireland Ltd. All rights reserved.

KW - Fast motor unit

KW - Nerve injury

KW - Neurotrophin

KW - Neurotrophin-3

KW - Slow motor unit

KW - Tyrosine kinase C

U2 - 10.1016/S0304-3940(02)00007-1

DO - 10.1016/S0304-3940(02)00007-1

M3 - Article

SN - 0304-3940

VL - 320

SP - 9

EP - 12

JO - Neuroscience letters

JF - Neuroscience letters

IS - 1-2

ER -

Differential tyrosine kinase C mRNA distribution in extensor digitorum longus and soleus motoneurons in adult rats: Effect of axotomy and neurotrophin-3 treatment

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Keywords

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