Differential uptake of photosynthetic and non-photosynthetic proteins by pea root plastids

Xianxi Yan; Sultan Khan; Toshiharu Hase; Michael J. Emes; Caroline G. Bowsher

doi:10.1016/j.febslet.2006.10.057

Differential uptake of photosynthetic and non-photosynthetic proteins by pea root plastids

Xianxi Yan, Sultan Khan, Toshiharu Hase, Michael J. Emes, Caroline G. Bowsher

Research output: Contribution to journal › Article › peer-review

Abstract

The photosynthetic proteins RuBiSCO, ferredoxin I and ferredoxin NADP+-oxidoreductase (pFNR) were efficiently imported into isolated pea chloroplasts but not into pea root plastids. By contrast non-photosynthetic ferredoxin III and heterotrophic FNR (hFNR) were efficiently imported into both isolated chloroplasts and root plastids. Chimeric ferredoxin I/III (transit peptide of ferredoxin I attached to the mature region of ferredoxin III) only imported into chloroplasts. Ferredoxin III/I (transit peptide of ferredoxin III attached to the mature region of ferredoxin I) imported into both chloroplasts and root plastids. This suggests that import depends on specific interactions between the transit peptide and the translocon apparatus. © 2006 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	6509-6512
Number of pages	3
Journal	FEBS Letters
Volume	580
Issue number	27
DOIs	https://doi.org/10.1016/j.febslet.2006.10.057
Publication status	Published - 27 Nov 2006

Keywords

Non-photosynthetic
Photosynthetic
Pisum sativum
Protein import
Root plastids
Transit peptide

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10.1016/j.febslet.2006.10.057

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title = "Differential uptake of photosynthetic and non-photosynthetic proteins by pea root plastids",

abstract = "The photosynthetic proteins RuBiSCO, ferredoxin I and ferredoxin NADP+-oxidoreductase (pFNR) were efficiently imported into isolated pea chloroplasts but not into pea root plastids. By contrast non-photosynthetic ferredoxin III and heterotrophic FNR (hFNR) were efficiently imported into both isolated chloroplasts and root plastids. Chimeric ferredoxin I/III (transit peptide of ferredoxin I attached to the mature region of ferredoxin III) only imported into chloroplasts. Ferredoxin III/I (transit peptide of ferredoxin III attached to the mature region of ferredoxin I) imported into both chloroplasts and root plastids. This suggests that import depends on specific interactions between the transit peptide and the translocon apparatus. {\textcopyright} 2006 Federation of European Biochemical Societies.",

keywords = "Non-photosynthetic, Photosynthetic, Pisum sativum, Protein import, Root plastids, Transit peptide",

author = "Xianxi Yan and Sultan Khan and Toshiharu Hase and Emes, {Michael J.} and Bowsher, {Caroline G.}",

year = "2006",

month = nov,

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doi = "10.1016/j.febslet.2006.10.057",

language = "English",

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TY - JOUR

T1 - Differential uptake of photosynthetic and non-photosynthetic proteins by pea root plastids

AU - Yan, Xianxi

AU - Khan, Sultan

AU - Hase, Toshiharu

AU - Emes, Michael J.

AU - Bowsher, Caroline G.

PY - 2006/11/27

Y1 - 2006/11/27

N2 - The photosynthetic proteins RuBiSCO, ferredoxin I and ferredoxin NADP+-oxidoreductase (pFNR) were efficiently imported into isolated pea chloroplasts but not into pea root plastids. By contrast non-photosynthetic ferredoxin III and heterotrophic FNR (hFNR) were efficiently imported into both isolated chloroplasts and root plastids. Chimeric ferredoxin I/III (transit peptide of ferredoxin I attached to the mature region of ferredoxin III) only imported into chloroplasts. Ferredoxin III/I (transit peptide of ferredoxin III attached to the mature region of ferredoxin I) imported into both chloroplasts and root plastids. This suggests that import depends on specific interactions between the transit peptide and the translocon apparatus. © 2006 Federation of European Biochemical Societies.

AB - The photosynthetic proteins RuBiSCO, ferredoxin I and ferredoxin NADP+-oxidoreductase (pFNR) were efficiently imported into isolated pea chloroplasts but not into pea root plastids. By contrast non-photosynthetic ferredoxin III and heterotrophic FNR (hFNR) were efficiently imported into both isolated chloroplasts and root plastids. Chimeric ferredoxin I/III (transit peptide of ferredoxin I attached to the mature region of ferredoxin III) only imported into chloroplasts. Ferredoxin III/I (transit peptide of ferredoxin III attached to the mature region of ferredoxin I) imported into both chloroplasts and root plastids. This suggests that import depends on specific interactions between the transit peptide and the translocon apparatus. © 2006 Federation of European Biochemical Societies.

KW - Non-photosynthetic

KW - Photosynthetic

KW - Pisum sativum

KW - Protein import

KW - Root plastids

KW - Transit peptide

U2 - 10.1016/j.febslet.2006.10.057

DO - 10.1016/j.febslet.2006.10.057

M3 - Article

VL - 580

SP - 6509

EP - 6512

JO - FEBS Letters

JF - FEBS Letters

IS - 27

ER -

Differential uptake of photosynthetic and non-photosynthetic proteins by pea root plastids

Abstract

Keywords

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