Direct comparison between Fӧrster Resonance Energy Transfer and Light-Induced Triplet–Triplet Electron Resonance Spectroscopy

Arnau Bertran, Laura Morbiato, Jack Sawyer, Chiara Dalla Torre, Derren J. Heyes, Sam Hay, Christiane R Timmel, Marilena Di Valentin, Marta De Zotti, Alice Bowen

Research output: Contribution to journalArticlepeer-review

Abstract

To carry out reliable and comprehensive structural investigations, the exploitation of different, complementary techniques is required. Here we report that dual triplet-spin/fluorescent labels enable the first parallel distance measurements by Electron Spin Resonance (ESR) and Förster Resonance Energy Transfer (FRET) on exactly the same molecules, with orthogonal chromophores, allowing for direct comparison. An improved light-induced triplet–triplet electron resonance method with two-color excitation is used, improving the signal-to-noise ratio of the data and yielding a distance distribution that provides greater insight than the single distance resulting from FRET.
Original languageEnglish
Pages (from-to)22859–22865
JournalJournal of the American Chemical Society
Volume145
Issue number42
Early online date15 Oct 2023
DOIs
Publication statusPublished - 25 Oct 2023

Keywords

  • Electron spin resonance (ESR)
  • electron paramagnetic resonance (EPR)
  • pulsed dipolar spectroscopy (PDS
  • Förster resonance energy transfer (FRET)
  • triplet state
  • distance measurement
  • spin-label
  • chromophore
  • porphyrin

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