Abstract
To carry out reliable and comprehensive structural investigations, the exploitation of different, complementary techniques is required. Here we report that dual triplet-spin/fluorescent labels enable the first parallel distance measurements by Electron Spin Resonance (ESR) and Förster Resonance Energy Transfer (FRET) on exactly the same molecules, with orthogonal chromophores, allowing for direct comparison. An improved light-induced triplet–triplet electron resonance method with two-color excitation is used, improving the signal-to-noise ratio of the data and yielding a distance distribution that provides greater insight than the single distance resulting from FRET.
Original language | English |
---|---|
Pages (from-to) | 22859–22865 |
Journal | Journal of the American Chemical Society |
Volume | 145 |
Issue number | 42 |
Early online date | 15 Oct 2023 |
DOIs | |
Publication status | Published - 25 Oct 2023 |
Keywords
- Electron spin resonance (ESR)
- electron paramagnetic resonance (EPR)
- pulsed dipolar spectroscopy (PDS
- Förster resonance energy transfer (FRET)
- triplet state
- distance measurement
- spin-label
- chromophore
- porphyrin
Fingerprint
Dive into the research topics of 'Direct comparison between Fӧrster Resonance Energy Transfer and Light-Induced Triplet–Triplet Electron Resonance Spectroscopy'. Together they form a unique fingerprint.Equipment
-
EPSRC National Research Facility for Electron Paramagnetic Resonance
Collison, D. (Academic lead), Mcinnes, E. (Academic lead), Tuna, F. (Academic lead), Bowen, A. (Academic lead), Shanmugam, M. (Senior Technical Specialist), Brookfield, A. (Technical Specialist), Fleming, E. (Other) & Cliff, M. (Platform Lead)
FSE ResearchFacility/equipment: Facility