Direct histological processing of EUS biopsies enables rapid molecular biomarker analysis for interventional pancreatic cancer trials.

Rebecca J Brais, Susan E Davies, Maria O'Donovan, Ben W Simpson, Natalie Cook, Walter C Darbonne, Sian Chilcott, Martijn P Lolkema, Albrecht Neesse, Michelle Lockley, Pippa G Corrie, Duncan I Jodrell, Raaj K Praseedom, Emmanuel L Huguet, Asif Jah, Neville V Jamieson, Frederic J de Sauvage, David A Tuveson, Nicholas R Carroll

    Research output: Contribution to journalArticlepeer-review

    Abstract

    OBJECTIVE: Current practice to diagnose pancreatic cancer is accomplished by endoscopic ultrasound guided fine needle aspiration (EUS-FNA) using a cytological approach. This method is time consuming and often fails to provide suitable specimens for modern molecular analyses. Here, we compare the cytological approach with direct formalin fixation of pancreatic EUS-FNA micro-cores and evaluate the potential to perform molecular biomarker analysis on these specimen. METHODS: 130 specimens obtained by EUS-FNA with a 22G needle were processed by the standard cytological approach and compared to a separate cohort of 130 specimens that were immediately formalin fixed to preserve micro-cores of tissue prior to routine histological processing. RESULTS: We found that direct formalin fixation significantly shortened the time required for diagnosis from 3.6 days to 2.9 days (p

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