Directed Evolution of an Amine Oxidase Possessing both Broad Substrate Specificity and High Enantioselectivity

Reuben Carr; Marina Alexeeva; Alexis Enright; Tom S C Eve; Michael J. Dawson; Nicholas J. Turner

doi:10.1002/anie.200352100

Directed Evolution of an Amine Oxidase Possessing both Broad Substrate Specificity and High Enantioselectivity

Reuben Carr, Marina Alexeeva, Alexis Enright, Tom S C Eve, Michael J. Dawson, Nicholas J. Turner

Research output: Contribution to journal › Article › peer-review

Abstract

Evolving enzymes: Directed evolution of the amine oxidase from Aspergillus niger, by using (S)-α-methylbenzylamine as the target substrate, resulted in a variant enzyme (Asn336Ser) possessing both broad substrate specificity and high S enantioselectivity towards a wide range of chiral amines (see scheme).

Original language	English
Pages (from-to)	4807-4810
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	42
Issue number	39
DOIs	https://doi.org/10.1002/anie.200352100
Publication status	Published - 13 Oct 2003

Keywords

Amines
Deracemization
Directed evolution
Enzymes

Access to Document

10.1002/anie.200352100

Cite this

@article{8e30fccfd923419e9007d5d648695a2f,

title = "Directed Evolution of an Amine Oxidase Possessing both Broad Substrate Specificity and High Enantioselectivity",

abstract = "Evolving enzymes: Directed evolution of the amine oxidase from Aspergillus niger, by using (S)-α-methylbenzylamine as the target substrate, resulted in a variant enzyme (Asn336Ser) possessing both broad substrate specificity and high S enantioselectivity towards a wide range of chiral amines (see scheme).",

keywords = "Amines, Deracemization, Directed evolution, Enzymes",

author = "Reuben Carr and Marina Alexeeva and Alexis Enright and Eve, {Tom S C} and Dawson, {Michael J.} and Turner, {Nicholas J.}",

year = "2003",

month = oct,

day = "13",

doi = "10.1002/anie.200352100",

language = "English",

volume = "42",

pages = "4807--4810",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley & Sons Ltd",

number = "39",

}

TY - JOUR

T1 - Directed Evolution of an Amine Oxidase Possessing both Broad Substrate Specificity and High Enantioselectivity

AU - Carr, Reuben

AU - Alexeeva, Marina

AU - Enright, Alexis

AU - Eve, Tom S C

AU - Dawson, Michael J.

AU - Turner, Nicholas J.

PY - 2003/10/13

Y1 - 2003/10/13

N2 - Evolving enzymes: Directed evolution of the amine oxidase from Aspergillus niger, by using (S)-α-methylbenzylamine as the target substrate, resulted in a variant enzyme (Asn336Ser) possessing both broad substrate specificity and high S enantioselectivity towards a wide range of chiral amines (see scheme).

AB - Evolving enzymes: Directed evolution of the amine oxidase from Aspergillus niger, by using (S)-α-methylbenzylamine as the target substrate, resulted in a variant enzyme (Asn336Ser) possessing both broad substrate specificity and high S enantioselectivity towards a wide range of chiral amines (see scheme).

KW - Amines

KW - Deracemization

KW - Directed evolution

KW - Enzymes

U2 - 10.1002/anie.200352100

DO - 10.1002/anie.200352100

M3 - Article

SN - 1433-7851

VL - 42

SP - 4807

EP - 4810

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 39

ER -

Directed Evolution of an Amine Oxidase Possessing both Broad Substrate Specificity and High Enantioselectivity

Abstract

Keywords

Access to Document

Fingerprint

Cite this