Discovery of a putative acetoin dehydrogenase complex in the hyperthermophilic archaeon Sulfolobus solfataricus

K. A P Payne; David W. Hough; Michael J. Danson

doi:10.1016/j.febslet.2010.02.037

Discovery of a putative acetoin dehydrogenase complex in the hyperthermophilic archaeon Sulfolobus solfataricus

K. A P Payne, David W. Hough, Michael J. Danson

Research output: Contribution to journal › Article › peer-review

Abstract

Like many other aerobic archaea, the hyperthermophile Sulfolobus solfataricus possesses a gene cluster encoding components of a putative 2-oxoacid dehydrogenase complex. In the current paper, we have cloned and expressed the first two genes of this cluster and demonstrate that the protein products form an α2β2 hetero-tetramer possessing the catalytic activity characteristic of the first component enzyme of an acetoin dehydrogenase multienzyme complex. This represents the first report of an acetoin multienzyme complex in archaea, and contrasts with the branched-chain 2-oxoacid dehydrogenase complex activities characterised in two other archaea, Thermoplasma acidophilum and Haloferax volcanii. © 2010 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	1231-1234
Number of pages	3
Journal	FEBS Letters
Volume	584
Issue number	6
DOIs	https://doi.org/10.1016/j.febslet.2010.02.037
Publication status	Published - Mar 2010

Keywords

2-Oxoacid dehydrogenase complex
Acetoin
Archaea
Metabolism
Thermophile

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10.1016/j.febslet.2010.02.037

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title = "Discovery of a putative acetoin dehydrogenase complex in the hyperthermophilic archaeon Sulfolobus solfataricus",

abstract = "Like many other aerobic archaea, the hyperthermophile Sulfolobus solfataricus possesses a gene cluster encoding components of a putative 2-oxoacid dehydrogenase complex. In the current paper, we have cloned and expressed the first two genes of this cluster and demonstrate that the protein products form an α2β2 hetero-tetramer possessing the catalytic activity characteristic of the first component enzyme of an acetoin dehydrogenase multienzyme complex. This represents the first report of an acetoin multienzyme complex in archaea, and contrasts with the branched-chain 2-oxoacid dehydrogenase complex activities characterised in two other archaea, Thermoplasma acidophilum and Haloferax volcanii. {\textcopyright} 2010 Federation of European Biochemical Societies.",

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author = "Payne, {K. A P} and Hough, {David W.} and Danson, {Michael J.}",

year = "2010",

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language = "English",

volume = "584",

pages = "1231--1234",

journal = "FEBS Letters",

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T1 - Discovery of a putative acetoin dehydrogenase complex in the hyperthermophilic archaeon Sulfolobus solfataricus

AU - Payne, K. A P

AU - Hough, David W.

AU - Danson, Michael J.

PY - 2010/3

Y1 - 2010/3

N2 - Like many other aerobic archaea, the hyperthermophile Sulfolobus solfataricus possesses a gene cluster encoding components of a putative 2-oxoacid dehydrogenase complex. In the current paper, we have cloned and expressed the first two genes of this cluster and demonstrate that the protein products form an α2β2 hetero-tetramer possessing the catalytic activity characteristic of the first component enzyme of an acetoin dehydrogenase multienzyme complex. This represents the first report of an acetoin multienzyme complex in archaea, and contrasts with the branched-chain 2-oxoacid dehydrogenase complex activities characterised in two other archaea, Thermoplasma acidophilum and Haloferax volcanii. © 2010 Federation of European Biochemical Societies.

AB - Like many other aerobic archaea, the hyperthermophile Sulfolobus solfataricus possesses a gene cluster encoding components of a putative 2-oxoacid dehydrogenase complex. In the current paper, we have cloned and expressed the first two genes of this cluster and demonstrate that the protein products form an α2β2 hetero-tetramer possessing the catalytic activity characteristic of the first component enzyme of an acetoin dehydrogenase multienzyme complex. This represents the first report of an acetoin multienzyme complex in archaea, and contrasts with the branched-chain 2-oxoacid dehydrogenase complex activities characterised in two other archaea, Thermoplasma acidophilum and Haloferax volcanii. © 2010 Federation of European Biochemical Societies.

KW - 2-Oxoacid dehydrogenase complex

KW - Acetoin

KW - Archaea

KW - Metabolism

KW - Thermophile

U2 - 10.1016/j.febslet.2010.02.037

DO - 10.1016/j.febslet.2010.02.037

M3 - Article

VL - 584

SP - 1231

EP - 1234

JO - FEBS Letters

JF - FEBS Letters

IS - 6

ER -

Discovery of a putative acetoin dehydrogenase complex in the hyperthermophilic archaeon Sulfolobus solfataricus

Abstract

Keywords

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