Discrepancies in Cancer Genomic Sequencing Highlight Opportunities for Driver Mutation Discovery.

Andrew Hudson, Timothy Yates, Yaoyong Li, Eleanor Trotter, Shameem Fawdar, Philip Chapman, Paul Lorigan, Andrew Biankin, Crispin J Miller, John Brognard

Research output: Contribution to journalArticlepeer-review


Cancer genome sequencing is being used at an increasing rate to identify actionable driver mutations that can inform therapeutic intervention strategies. A comparison of two of the most prominent cancer genome sequencing databases from different institutes (Cancer Cell Line Encyclopedia and Catalogue of Somatic Mutations in Cancer) revealed marked discrepancies in the detection of missense mutations in identical cell lines (57.38% conformity). The main reason for this discrepancy is inadequate sequencing of GC-rich areas of the exome. We have therefore mapped over 400 regions of consistent inadequate sequencing (cold-spots) in known cancer-causing genes and kinases in 368, of which neither institute finds mutations. We demonstrate, using a newly identified PAK4 mutation as proof of principle, that specific targeting and sequencing of these GC-rich cold-spot regions can lead to the identification of novel driver mutations in known tumor suppressors and oncogenes. We highlight that cross-referencing between genomic databases is required to comprehensively assess genomic alterations in commonly used cell lines and that there are still significant opportunities to identify novel drivers of tumorigenesis in poorly sequenced areas of the exome. Finally, we assess other reasons for the observed discrepancy, such as variations in dbSNP filtering and the acquisition/loss of mutations, to give explanations as to why there is a discrepancy in pharmacogenomic studies, given recent concerns with poor reproducibility of data. Cancer Res; 74(22); 1-7. ©2014 AACR.
Original languageEnglish
JournalCancer Research
Issue number22
Publication statusPublished - Nov 2014


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