Dissolved oxygen alteration of the spectrophotometric analysis and quantification of nucleic acid solutions

Rupak Doshi, Philip J R Day, Nicola Tirelli

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Nucleic acids are routinely and readily analyses using the A260/A280 ratio, although this method is known to be prone to erroneous results owing to contaminants in solution that absorb at similar wavelengths. The aim of the present review, while highlighting the problems and alternatives of using UV spectrophotometry for nucleic acid measurements, is to bring forth an observational result from our recent studies, namely that DO (dissolved oxygen) and nitrogen can alter the A260 of aqueous DNA solutions. Our finding is of importance because DO is highly variable between protocols and storage conditions of DNA preparations. The physicochemical nature of the oxygen-DNA interactions is briefly discussed. © The Authors Journal compilation © 2009 Biochemical Society.
    Original languageEnglish
    Pages (from-to)466-470
    Number of pages4
    JournalBiochemical Society Transactions
    Volume37
    Issue number2
    DOIs
    Publication statusPublished - 2009

    Keywords

    • Dissolved oxygen/nitrogen
    • DNA purity
    • DNA quantification
    • Nucleic acid measurement
    • Oxygen-DNA interaction
    • UV spectrophotometry

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