Distribution of 7-methylguanine and of replication sites in the different kinetic classes of DNA from rats treated with dimethylnitrosamine

A. I. Galbraith; M. R. Chapleo; R. F. Itzhaki

Distribution of 7-methylguanine and of replication sites in the different kinetic classes of DNA from rats treated with dimethylnitrosamine

A. I. Galbraith, M. R. Chapleo, R. F. Itzhaki

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Abstract

The distribution of 7-methylguanine in the families of repetitive and unique sequences of rat liver chromatin DNA has been studied using the technique of DNA-DNA reassociation. Rats were injected with di[14C]methylnitrosamine and chromatin DNA was prepared 3 h later. The distribution of 7-methylguanine was found to be random between these classes of DNA. We have also studied chromatin DNA from rats treated with unlabelled DMN plus [3H]thymidine in this way, in order to find if DMN affects DNA synthesis within any one kinetic class. Our results suggest that there is no difference in the extent of synthesis between these classes.

Original language	English
Pages (from-to)	3357-3364
Number of pages	7
Journal	Nucleic acids research.
Volume	5
Issue number	9
Publication status	Published - 1978

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title = "Distribution of 7-methylguanine and of replication sites in the different kinetic classes of DNA from rats treated with dimethylnitrosamine",

abstract = "The distribution of 7-methylguanine in the families of repetitive and unique sequences of rat liver chromatin DNA has been studied using the technique of DNA-DNA reassociation. Rats were injected with di[14C]methylnitrosamine and chromatin DNA was prepared 3 h later. The distribution of 7-methylguanine was found to be random between these classes of DNA. We have also studied chromatin DNA from rats treated with unlabelled DMN plus [3H]thymidine in this way, in order to find if DMN affects DNA synthesis within any one kinetic class. Our results suggest that there is no difference in the extent of synthesis between these classes.",

author = "Galbraith, {A. I.} and Chapleo, {M. R.} and Itzhaki, {R. F.}",

year = "1978",

language = "English",

volume = "5",

pages = "3357--3364",

journal = "Nucleic acids research.",

issn = "1362-4962",

publisher = "Oxford University Press",

number = "9",

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TY - JOUR

T1 - Distribution of 7-methylguanine and of replication sites in the different kinetic classes of DNA from rats treated with dimethylnitrosamine

AU - Galbraith, A. I.

AU - Chapleo, M. R.

AU - Itzhaki, R. F.

PY - 1978

Y1 - 1978

N2 - The distribution of 7-methylguanine in the families of repetitive and unique sequences of rat liver chromatin DNA has been studied using the technique of DNA-DNA reassociation. Rats were injected with di[14C]methylnitrosamine and chromatin DNA was prepared 3 h later. The distribution of 7-methylguanine was found to be random between these classes of DNA. We have also studied chromatin DNA from rats treated with unlabelled DMN plus [3H]thymidine in this way, in order to find if DMN affects DNA synthesis within any one kinetic class. Our results suggest that there is no difference in the extent of synthesis between these classes.

AB - The distribution of 7-methylguanine in the families of repetitive and unique sequences of rat liver chromatin DNA has been studied using the technique of DNA-DNA reassociation. Rats were injected with di[14C]methylnitrosamine and chromatin DNA was prepared 3 h later. The distribution of 7-methylguanine was found to be random between these classes of DNA. We have also studied chromatin DNA from rats treated with unlabelled DMN plus [3H]thymidine in this way, in order to find if DMN affects DNA synthesis within any one kinetic class. Our results suggest that there is no difference in the extent of synthesis between these classes.

M3 - Article

C2 - 704360

SN - 1362-4962

VL - 5

SP - 3357

EP - 3364

JO - Nucleic acids research.

JF - Nucleic acids research.

IS - 9

ER -

Distribution of 7-methylguanine and of replication sites in the different kinetic classes of DNA from rats treated with dimethylnitrosamine

Abstract

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