DNA damage controlled production of green fluorescent protein in genotoxicity assessment

Richard M Walmsley; M G Barker; D A Bailey; N J Billinton; W D Heyer; A W Knight; P J Fielden; N J Goddard

DNA damage controlled production of green fluorescent protein in genotoxicity assessment

Richard M Walmsley, M G Barker, D A Bailey, N J Billinton, W D Heyer, A W Knight, P J Fielden, N J Goddard

Research output: Contribution to journal › Article › peer-review

Abstract

Most DNA damage is repaired, so the activity of inducible DNA repair systems provides a good est. of the genotoxicity of a cell's environment. Existing genotoxicity tests (such as Ames test and SOS chromotest) exploit bacteria (prokaryotes), though their evolutionary distance from humans can lead to false pos. or neg. results, so we are developing a eukaryotic (yeast) system. In our system the promoter of the DNA damage-induced yeast gene RAD54 is used to drive prodn. of Green Fluorescent Protein. Results of a development program will be presented, in which the problems of low promoter activity and high background fluorescence were minimised. A microtitre plate test, and an online monitor have been developed which provide complementary results within 4-5 h. [on SciFinder (R)]

Original language	English
Pages (from-to)	BIOT-009
Journal	Book of Abstracts, 217th ACS National Meeting, Anaheim, Calif., March 21-25
Publication status	Published - 1999

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title = "DNA damage controlled production of green fluorescent protein in genotoxicity assessment",

abstract = "Most DNA damage is repaired, so the activity of inducible DNA repair systems provides a good est. of the genotoxicity of a cell's environment. Existing genotoxicity tests (such as Ames test and SOS chromotest) exploit bacteria (prokaryotes), though their evolutionary distance from humans can lead to false pos. or neg. results, so we are developing a eukaryotic (yeast) system. In our system the promoter of the DNA damage-induced yeast gene RAD54 is used to drive prodn. of Green Fluorescent Protein. Results of a development program will be presented, in which the problems of low promoter activity and high background fluorescence were minimised. A microtitre plate test, and an online monitor have been developed which provide complementary results within 4-5 h. [on SciFinder (R)]",

author = "Walmsley, {Richard M} and Barker, {M G} and Bailey, {D A} and Billinton, {N J} and Heyer, {W D} and Knight, {A W} and Fielden, {P J} and Goddard, {N J}",

note = "Department of Biomolecular Sciences,UMIST,Manchester,UK. Conference; Meeting Abstract",

year = "1999",

language = "English",

pages = "BIOT--009",

journal = "Book of Abstracts, 217th ACS National Meeting, Anaheim, Calif., March 21-25",

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TY - JOUR

T1 - DNA damage controlled production of green fluorescent protein in genotoxicity assessment

AU - Walmsley, Richard M

AU - Barker, M G

AU - Bailey, D A

AU - Billinton, N J

AU - Heyer, W D

AU - Knight, A W

AU - Fielden, P J

AU - Goddard, N J

N1 - Department of Biomolecular Sciences,UMIST,Manchester,UK. Conference; Meeting Abstract

PY - 1999

Y1 - 1999

N2 - Most DNA damage is repaired, so the activity of inducible DNA repair systems provides a good est. of the genotoxicity of a cell's environment. Existing genotoxicity tests (such as Ames test and SOS chromotest) exploit bacteria (prokaryotes), though their evolutionary distance from humans can lead to false pos. or neg. results, so we are developing a eukaryotic (yeast) system. In our system the promoter of the DNA damage-induced yeast gene RAD54 is used to drive prodn. of Green Fluorescent Protein. Results of a development program will be presented, in which the problems of low promoter activity and high background fluorescence were minimised. A microtitre plate test, and an online monitor have been developed which provide complementary results within 4-5 h. [on SciFinder (R)]

AB - Most DNA damage is repaired, so the activity of inducible DNA repair systems provides a good est. of the genotoxicity of a cell's environment. Existing genotoxicity tests (such as Ames test and SOS chromotest) exploit bacteria (prokaryotes), though their evolutionary distance from humans can lead to false pos. or neg. results, so we are developing a eukaryotic (yeast) system. In our system the promoter of the DNA damage-induced yeast gene RAD54 is used to drive prodn. of Green Fluorescent Protein. Results of a development program will be presented, in which the problems of low promoter activity and high background fluorescence were minimised. A microtitre plate test, and an online monitor have been developed which provide complementary results within 4-5 h. [on SciFinder (R)]

M3 - Article

SP - BIOT-009

JO - Book of Abstracts, 217th ACS National Meeting, Anaheim, Calif., March 21-25

JF - Book of Abstracts, 217th ACS National Meeting, Anaheim, Calif., March 21-25

ER -

DNA damage controlled production of green fluorescent protein in genotoxicity assessment

Abstract

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