DNA-mediated transformation of fungi

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The advent of techniques for manipulating and isolating genes from plant pathogenic fungi has lead to a rapid increase in our understanding of how these organisms cause disease I1l. An important factor in these advances has been the ability to re-introduce homologous or heterologous genes into these organisms. Transformation systems for model fungi such as yeast, Neurospora crassa, and Aspergillus nidulans. have existed since the 1970s and these techniques are being adapted for use in a growing array of pathogens. This chapter concentrates on techniques developed for pathogenic fungal species, but the techniques have often been found to be broadly applicable among fungal species. The vast majority of transformation protocols for filamentous fungi are based on permeabilizing cell membranes with polyethylene glycol IPEGI, or electroporation. Both methods typically require preparing protoplasts, or osmotically-sensitive cells, prior to transformation I2I Ia detailed review of fungal transformation systems is given in ref. 3I. Many phytopathogenic fungi have been transformed Isee Table 1) using these methods, however the obligately parasitic nature of some of these organisms has rendered them difficult to transform. Methods used for transformation of such'difficult'pathogens, such as particle bombardment and Agrobacterium-mediated DNA transfer I4I are therefore also included.
Original languageEnglish
Title of host publicationMolecular and cellular biology of filamentous fungi
Subtitle of host publicationA practical approach
EditorsNick Talbot
PublisherOxford University Press
Number of pages14
ISBN (Print)9780199638376
Publication statusPublished - 19 Apr 2001

Publication series

NamePractical Approach


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