DNA typing of epidemiologically-related isolates of Aspergillus fumigatus

M. Birch; N. Nolard; G. S. Shankland; D. W. Denning

doi:10.1017/S0950268800052018

DNA typing of epidemiologically-related isolates of Aspergillus fumigatus

M. Birch, N. Nolard, G. S. Shankland, D. W. Denning

Division of Immunology, Immunity to Infection and Respiratory Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Invasive aspergillosis is often nosocomially acquired and carries a high mortality. Molecular typing methods to discriminate isolates have now been developed. Using simple restriction endonuclease (Sal1 and Xho1) digestion of total genomic DNA, we have typed 25 epidemiologically-related isolates of A. fumigatus from six hospital episodes of invasive aspergillosis. Eight DNA types were found and in each case the DNA type matched precisely the epidemiological data. Thus DNA typing of A. fumigatus can provide the means to match isolates from linked sources and distinguish isolates from diverse origins.

Original language	English
Pages (from-to)	161-168
Number of pages	8
Journal	Epidemiology and infection
Volume	114
Issue number	1
DOIs	https://doi.org/10.1017/S0950268800052018
Publication status	Published - Feb 1995

Keywords

Aspergillosis

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10.1017/S0950268800052018

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abstract = "Invasive aspergillosis is often nosocomially acquired and carries a high mortality. Molecular typing methods to discriminate isolates have now been developed. Using simple restriction endonuclease (Sal1 and Xho1) digestion of total genomic DNA, we have typed 25 epidemiologically-related isolates of A. fumigatus from six hospital episodes of invasive aspergillosis. Eight DNA types were found and in each case the DNA type matched precisely the epidemiological data. Thus DNA typing of A. fumigatus can provide the means to match isolates from linked sources and distinguish isolates from diverse origins.",

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author = "M. Birch and N. Nolard and Shankland, \{G. S.\} and Denning, \{D. W.\}",

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AU - Denning, D. W.

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AB - Invasive aspergillosis is often nosocomially acquired and carries a high mortality. Molecular typing methods to discriminate isolates have now been developed. Using simple restriction endonuclease (Sal1 and Xho1) digestion of total genomic DNA, we have typed 25 epidemiologically-related isolates of A. fumigatus from six hospital episodes of invasive aspergillosis. Eight DNA types were found and in each case the DNA type matched precisely the epidemiological data. Thus DNA typing of A. fumigatus can provide the means to match isolates from linked sources and distinguish isolates from diverse origins.

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DNA typing of epidemiologically-related isolates of Aspergillus fumigatus

Abstract

Keywords

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