Drosophila shaking-B protein forms gap junctions in paired Xenopus oocytes

Pauline Phelan, Lucy A. Stebbings, Richard A. Baines, Jonathan P. Bacon, Jane A. Davies, Chris Ford

    Research output: Contribution to journalArticlepeer-review

    Abstract

    In most multicellular organisms direct cell-cell communication is mediated by the intercellular channels of gap junctions. These channels allow the exchange of ions and molecules that are believed to be essential for cell signalling development and in some differentiated tissues. Proteins called connexins, which are products of a multigene family are the structural components of vertebrate gap junctions. Surprisingly, molecular homologues of the connexins have not been described in any invertebrate. A separate gene family, which includes the Drosophila genes shaking-B and l(1)ogre, and the Caenorhabditis elegans genes unc-7 and eat-5, encodes transmembrane proteins with a predicted structure similar to that of the connexins. shaking-B and eat-5 are required for the formation of functional gap junctions. To test directly whether Shaking-B is a channel protein, we expressed it is paired Xenopus oocytes. Here we show that Shaking-B localizes to the membrane, and that its presence induces the formation of functional intercellular channels. To our knowledge, this is the first structural component of an invertebrate gap junction to be characterized.
    Original languageEnglish
    Pages (from-to)181-184
    Number of pages3
    JournalNature
    Volume391
    Issue number6663
    DOIs
    Publication statusPublished - 8 Jan 1998

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