Abstract
Sustained elevated levels of reactive oxygen species (ROS) have been shown to be
essential for regeneration in many organisms. This has been shown primarily via the use of
pharmacological inhibitors targeting the family of NAPDH oxidases (NOXes). To identify the
specific Noxes involved in ROS production during adult caudal fin regeneration in zebrafish,
we generated nox mutants for duox, nox5 and cyba (a key subunit of Noxes 1-4) and
crossed these lines with a transgenic line ubiquitously expressing HyPer, which permits the
measurement of ROS levels. Homozygous duox mutants had the greatest effect on ROS
levels and rate of fin regeneration amongst the single mutants. However, duox:cyba double
mutants showed a greater effect on fin regeneration than the single duox mutants,
suggesting that Nox1-4 also play a role during regeneration. This work also serendipitously
found that ROS levels in amputated adult zebrafish fins oscillate with a circadian rhythm.
essential for regeneration in many organisms. This has been shown primarily via the use of
pharmacological inhibitors targeting the family of NAPDH oxidases (NOXes). To identify the
specific Noxes involved in ROS production during adult caudal fin regeneration in zebrafish,
we generated nox mutants for duox, nox5 and cyba (a key subunit of Noxes 1-4) and
crossed these lines with a transgenic line ubiquitously expressing HyPer, which permits the
measurement of ROS levels. Homozygous duox mutants had the greatest effect on ROS
levels and rate of fin regeneration amongst the single mutants. However, duox:cyba double
mutants showed a greater effect on fin regeneration than the single duox mutants,
suggesting that Nox1-4 also play a role during regeneration. This work also serendipitously
found that ROS levels in amputated adult zebrafish fins oscillate with a circadian rhythm.
| Original language | English |
|---|---|
| Journal | iScience |
| Publication status | Published - 2023 |