Dynamic analysis of STAT6 signalling in living cells

Glyn Nelson; Geraint J C Wilde; David G. Spiller; Elaine Sullivan; John F. Unitt; Michael R H White

doi:10.1016/S0014-5793(02)03672-4

Dynamic analysis of STAT6 signalling in living cells

Glyn Nelson, Geraint J C Wilde, David G. Spiller, Elaine Sullivan, John F. Unitt, Michael R H White

Research output: Contribution to journal › Article › peer-review

Abstract

Functional activity of N- and C-terminal fluorescent fusion proteins between STAT6 and EGFP was demonstrated through IL-4-dependent transcriptional activation and nuclear translocation. The N-terminal (EGFP-STAT6) fusion protein appeared to be more active than the C-terminal fusion. In HEK-293 cells both fusion proteins formed fluorescent nuclear foci following IL-4 stimulation, but in HeLa cells nuclear accumulation was homogeneous. Stimulation of the NF-κB pathway through TNFα treatment, or expression of p65-EGFP fusion protein, repressed both basal STAT6-dependent transcriptional activity and the extent of activation in response to IL-4. This indicates a novel mechanism of inhibition of STAT6 signalling by NF-κB activation. © 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	188-192
Number of pages	4
Journal	FEBS Letters
Volume	532
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(02)03672-4
Publication status	Published - 4 Dec 2002

Keywords

Confocal microscopy
Green fluorescent protein
NF-κB
STAT6

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10.1016/S0014-5793(02)03672-4

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title = "Dynamic analysis of STAT6 signalling in living cells",

abstract = "Functional activity of N- and C-terminal fluorescent fusion proteins between STAT6 and EGFP was demonstrated through IL-4-dependent transcriptional activation and nuclear translocation. The N-terminal (EGFP-STAT6) fusion protein appeared to be more active than the C-terminal fusion. In HEK-293 cells both fusion proteins formed fluorescent nuclear foci following IL-4 stimulation, but in HeLa cells nuclear accumulation was homogeneous. Stimulation of the NF-κB pathway through TNFα treatment, or expression of p65-EGFP fusion protein, repressed both basal STAT6-dependent transcriptional activity and the extent of activation in response to IL-4. This indicates a novel mechanism of inhibition of STAT6 signalling by NF-κB activation. {\textcopyright} 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.",

keywords = "Confocal microscopy, Green fluorescent protein, NF-κB, STAT6",

author = "Glyn Nelson and Wilde, {Geraint J C} and Spiller, {David G.} and Elaine Sullivan and Unitt, {John F.} and White, {Michael R H}",

year = "2002",

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day = "4",

doi = "10.1016/S0014-5793(02)03672-4",

language = "English",

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journal = "FEBS Letters",

publisher = "John Wiley & Sons Ltd",

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TY - JOUR

T1 - Dynamic analysis of STAT6 signalling in living cells

AU - Nelson, Glyn

AU - Wilde, Geraint J C

AU - Spiller, David G.

AU - Sullivan, Elaine

AU - Unitt, John F.

AU - White, Michael R H

PY - 2002/12/4

Y1 - 2002/12/4

N2 - Functional activity of N- and C-terminal fluorescent fusion proteins between STAT6 and EGFP was demonstrated through IL-4-dependent transcriptional activation and nuclear translocation. The N-terminal (EGFP-STAT6) fusion protein appeared to be more active than the C-terminal fusion. In HEK-293 cells both fusion proteins formed fluorescent nuclear foci following IL-4 stimulation, but in HeLa cells nuclear accumulation was homogeneous. Stimulation of the NF-κB pathway through TNFα treatment, or expression of p65-EGFP fusion protein, repressed both basal STAT6-dependent transcriptional activity and the extent of activation in response to IL-4. This indicates a novel mechanism of inhibition of STAT6 signalling by NF-κB activation. © 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

AB - Functional activity of N- and C-terminal fluorescent fusion proteins between STAT6 and EGFP was demonstrated through IL-4-dependent transcriptional activation and nuclear translocation. The N-terminal (EGFP-STAT6) fusion protein appeared to be more active than the C-terminal fusion. In HEK-293 cells both fusion proteins formed fluorescent nuclear foci following IL-4 stimulation, but in HeLa cells nuclear accumulation was homogeneous. Stimulation of the NF-κB pathway through TNFα treatment, or expression of p65-EGFP fusion protein, repressed both basal STAT6-dependent transcriptional activity and the extent of activation in response to IL-4. This indicates a novel mechanism of inhibition of STAT6 signalling by NF-κB activation. © 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

KW - Confocal microscopy

KW - Green fluorescent protein

KW - NF-κB

KW - STAT6

U2 - 10.1016/S0014-5793(02)03672-4

DO - 10.1016/S0014-5793(02)03672-4

M3 - Article

VL - 532

SP - 188

EP - 192

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Dynamic analysis of STAT6 signalling in living cells

Abstract

Keywords

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