Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation

Edward Brown; Trevor McKee; Emmanuelle DiTomaso; Alain Pluen; Brian Seed; Yves Boucher; Rakesh K. Jain

doi:10.1038/nm879

Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation

Edward Brown, Trevor McKee, Emmanuelle DiTomaso, Alain Pluen, Brian Seed, Yves Boucher, Rakesh K. Jain

Research output: Contribution to journal › Article › peer-review

Abstract

The content and structure of collagen is essential in governing the delivery of therapeutic molecules in tumors. Thus, simple histological staining of tumor tissue biopsies for collagen could be used to assess the accessibility of molecular therapeutics in tumors. Here we show that it is possible to optically image fibrillar collagen in tumors growing in mice using second-harmonic generation (SHG). Using this noninvasive technique, we estimated relative diffusive hindrance, quantified the dynamics of collagen modification after pharmacologic intervention and provided mechanistic insight into improved diffusive transport induced by the hormone relaxin. This technology could offer basic scientists and clinicians an enhanced ability to estimate the relative penetrabilities of molecular therapeutics.

Original language	English
Pages (from-to)	796-800
Number of pages	4
Journal	Nature Medicine
Volume	9
Issue number	6
DOIs	https://doi.org/10.1038/nm879
Publication status	Published - 1 Jun 2003

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10.1038/nm879

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abstract = "The content and structure of collagen is essential in governing the delivery of therapeutic molecules in tumors. Thus, simple histological staining of tumor tissue biopsies for collagen could be used to assess the accessibility of molecular therapeutics in tumors. Here we show that it is possible to optically image fibrillar collagen in tumors growing in mice using second-harmonic generation (SHG). Using this noninvasive technique, we estimated relative diffusive hindrance, quantified the dynamics of collagen modification after pharmacologic intervention and provided mechanistic insight into improved diffusive transport induced by the hormone relaxin. This technology could offer basic scientists and clinicians an enhanced ability to estimate the relative penetrabilities of molecular therapeutics.",

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AU - McKee, Trevor

AU - DiTomaso, Emmanuelle

AU - Pluen, Alain

AU - Seed, Brian

AU - Boucher, Yves

AU - Jain, Rakesh K.

PY - 2003/6/1

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AB - The content and structure of collagen is essential in governing the delivery of therapeutic molecules in tumors. Thus, simple histological staining of tumor tissue biopsies for collagen could be used to assess the accessibility of molecular therapeutics in tumors. Here we show that it is possible to optically image fibrillar collagen in tumors growing in mice using second-harmonic generation (SHG). Using this noninvasive technique, we estimated relative diffusive hindrance, quantified the dynamics of collagen modification after pharmacologic intervention and provided mechanistic insight into improved diffusive transport induced by the hormone relaxin. This technology could offer basic scientists and clinicians an enhanced ability to estimate the relative penetrabilities of molecular therapeutics.

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JO - Nature Medicine

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Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation

Abstract

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