Dynein is required for receptor sorting and the morphogenesis of early endosomes

    Research output: Contribution to journalArticlepeer-review


    The early endosome is organised into domains to ensure the separation of cargo. Activated mitogenic receptors, such as epidermal growth factor (EGF) receptor, are concentrated into vacuoles enriched for the small GTPase Rab5, which progressively exclude nutrient receptors, such as transferrin receptor, into neighbouring tubules. These vacuoles become enlarged, increase their content of intralumenal vesicles as EGF receptor is sorted from the limiting membrane, and eventually mature to late endosomes. Maturation is governed by the loss of Rab5 and is accompanied by the movement of endosomes along microtubules towards the cell centre. Here, we show that EGF relocates to the cell centre in a dynein-dependent fashion, concomitant with the sorting away of transferrin receptor, although it remains in Rab5-positive early endosomes. When dynein function is acutely disrupted, efficient recycling of transferrin from EGF-containing endosomes is retarded, loss of Rab5 is slowed and endosome enlargement is reduced.
    Original languageEnglish
    Pages (from-to)113-120
    Number of pages7
    JournalNature Cell Biology
    Issue number1
    Publication statusPublished - Jan 2007


    Dive into the research topics of 'Dynein is required for receptor sorting and the morphogenesis of early endosomes'. Together they form a unique fingerprint.

    Cite this