Effect of endogenous and exogenous nitric oxide on calcium sparks as targets for vasodilation in rat cerebral artery

Maurizio Mandalà, Thomas J. Heppner, Adrian D. Bonev, Mark T. Nelson

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The potent vasodilator nitric oxide (NO), produced mainly by the endothelium, acts through a BKCa-dependent mechanism to increase the frequency of calcium sparks (Ca2+ sparks) in myocyte isolated from rat cerebral arteries. Our present aim has been to assess the role of endogenous and exogenous NO on the Ca2+ sparks through ryanodine-sensitive channels in the sarcoplasmic reticulum of an intact artery. Calcium sparks, detected with fluo-4 and laser scanning confocal microscopy, were examined in isolated pressurized rat posterior cerebral arteries with (intact) and without endothelium (denuded). Addition of the NO donor, DEA-NONOate (N-(2-aminoethyl)-N-(2-hydroxy-2-nitrosohydrazino)-1,2-ethylenediamine), did not change the amplitude and frequency of Ca2+ sparks in the intact artery. However, inhibition of nitric oxide synthase with N-ω-nitro-l-arginine or removal of endothelium reduced Ca2+ sparks frequency by about 50%. Under these conditions (i.e., absence of endogenous NO production), DEA-NONOate, increased Ca2+ spark frequency 3- to 4-fold. These results suggest that endothelial NO modulates local Ca2+ release events in the arterial smooth muscle and that this mechanism may contribute to the actions of nitrovasodilators. © 2006 Elsevier Inc. All rights reserved.
    Original languageEnglish
    Pages (from-to)104-109
    Number of pages5
    JournalNitric Oxide - Biology and Chemistry
    Volume16
    Issue number1
    DOIs
    Publication statusPublished - Feb 2007

    Keywords

    • Calcium sparks
    • Cerebral arteries
    • Nitric oxide

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