TY - JOUR
T1 - Effect of growth substrate and electron donor on hydrogenation of carbon-carbon double bonds by Acetobacterium woodii
AU - Stephens, Gillian
AU - Davies, E. Timothy
AU - Stephens, Gillian M.
N1 - CAN 129:135257 16-9 Fermentation and Bioindustrial Chemistry Department of Chemical Engineering, UMIST,Manchester,UK. Journal 0141-0229 written in English. 57-48-7 (Fructose); 67-56-1 (Methanol); 90-50-6 (3,4,5-Trimethoxycinnamic acid); 331-39-5; 25173-72-2 Role: BSU (Biological study, unclassified), BIOL (Biological study) (effect of growth substrate and electron donor on hydrogenation of carbon-carbon double bonds by Acetobacterium woodii)
PY - 1998/7
Y1 - 1998/7
N2 - Acetobacterium woodii was grown on fructose, methanol, 3,4,5-trimethoxycinnamate, or 3-(3,4,5-trimethoxyphenyl)-propanoate in the prescence or abscence of caffeate. The cells were harvested and tested for their ability to hydrogenate C=C bonds using caffeate as a model substrate. Except for cells grown on methanol, the growth substrate was a poor electron donor for the biotransformation. 3-(3,4,55-Trimethoxyphenyl)-propanoate was the best donor for fructose-grown cells while fructose was the best donor for cells grown on methoxy-substituted aromatics. Caffeate reductase activity was inducible by caffeate or trimethoxycinnamate. The highest rates of caffeate reduction were obtained using cells which had been grown on 3,4,5-trimethoxycinnamate plus caffeate and by providing fructose as an electron donor.
AB - Acetobacterium woodii was grown on fructose, methanol, 3,4,5-trimethoxycinnamate, or 3-(3,4,5-trimethoxyphenyl)-propanoate in the prescence or abscence of caffeate. The cells were harvested and tested for their ability to hydrogenate C=C bonds using caffeate as a model substrate. Except for cells grown on methanol, the growth substrate was a poor electron donor for the biotransformation. 3-(3,4,55-Trimethoxyphenyl)-propanoate was the best donor for fructose-grown cells while fructose was the best donor for cells grown on methoxy-substituted aromatics. Caffeate reductase activity was inducible by caffeate or trimethoxycinnamate. The highest rates of caffeate reduction were obtained using cells which had been grown on 3,4,5-trimethoxycinnamate plus caffeate and by providing fructose as an electron donor.
KW - Hydrogenation (biol.
KW - effect of growth substrate and electron donor on hydrogenation of carbon-carbon double bonds by Acetobacterium woodii)
KW - Acetobacterium woodii
KW - Double bond
KW - Electron donors (effect of growth substrate and electron donor on hydrogenation of carbon-carbon double bonds by Acetobacterium woodii)
KW - Acetobacterium carbon double bond hydrogenation trimethoxyphenylpropanoate
M3 - Article
SN - 1879-0909
VL - 23
SP - 129
EP - 132
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 1-2
ER -