Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets

Reinhard Bauer, Peter Brust, Bernd Walter, Gerd Vorwieger, Ralf Bergmann, Elsayed Elhalag, Anne Fritz, Jörg Steinbach, Frank Füchtner, Rainer Hinz, Ulrich Zwiener, Bernd Johannsen

    Research output: Contribution to journalArticlepeer-review


    There is evidence that the dopaminergic system is sensitive to altered po2 in the immature brain. However, the respective enzyme activities have not been measured in the living neonatal brain together with brain oxidative metabolism. Therefore 18F-labelled 6-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) together with positron emission tomography (PET) was used to estimate the activity of the aromatic amino acid decarboxylase (AADC) in the brain of fifteen newborn piglets (2-5 days old). Two PET scans were performed in each piglet. Eleven animals underwent a period of normoxia and moderate hypoxia/hypercapnia (H/H). The remaining four animals were used as an untreated control group. Simultaneously, the brain tissue po2 was recorded, the regional cerebral blood flow (CBF) was measured with colored microspheres and the cerebral metabolic rate of oxygen (CMRO2) was determined. In addition, in four untreated and six H/H treated piglets the relative amounts of fluorodopamine and the respective metabolites were determined in brain tissue samples using HPLC analysis. H/H conditions were induced by lowering the inspired fraction of oxygen from 0.35 to 0.10 and adding CO2 to the inspired gas resulting in an arterial pco2 between 74 and 79 mmHg. H/H elicited a more than 3-fold increase of the CBF (P
    Original languageEnglish
    Pages (from-to)23-33
    Number of pages10
    JournalBrain research
    Issue number1
    Publication statusPublished - 26 Apr 2002


    • Aromatic amino acid decarboxylase
    • Colored microsphere
    • Dopamine metabolism
    • FDOPA
    • Newborn piglet
    • Positron emission tomography


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