Abstract
Hypoxic injury is a major cause of tubular necrosis in the corticomedullary junction of isolated perfused kidneys, and is ameliorated by inhibitors of active reabsorption, such as frusemide. Our objective was to determine whether frusemide has a similar effect on hypothermically stored transplanted kidneys and whether this effect is modulated by impermeant solutes included in the preservation solution. The effect of frusemide on cytochrome oxidase (cyt aa3) oxidation, renal hemodynamics, and morphology was investigated in the New Zealand White rabbit renal autograft model using near-infrared spectroscopy and light microscopy. A total of 30 kidneys were autografted in six groups. Kidneys were transplanted with or without frusemide either (1) without storage (groups 1 and 2) or after 72 hr of storage in: (2) hypertonic citrate containing mannitol (groups 3 and 4); and (3) hypertonic citrate containing polyethylene glycol (PEG) (groups 5 and 6). In unstored transplanted kidneys, frusemide infusion stimulated a significant (P
| Original language | English |
|---|---|
| Pages (from-to) | 575-582 |
| Number of pages | 7 |
| Journal | Transplantation |
| Volume | 62 |
| Issue number | 5 |
| Publication status | Published - 15 Sept 1996 |
Keywords
- Animals
- pharmacology: Diuretics
- Drug Interactions
- metabolism: Electron Transport Complex IV
- Female
- pharmacology: Furosemide
- metabolism: Hemoglobins
- Hypothermia, Induced
- blood supply: Kidney
- methods: Kidney Transplantation
- Mannitol
- methods: Organ Preservation
- Organ Preservation Solutions
- metabolism: Oxyhemoglobins
- Perfusion
- Polyethylene Glycols
- Rabbits