TY - JOUR
T1 - Effect of slow and ultra-rapid freezing on cell surface antigens of 8-cell mouse embryos.
AU - Wood, M. J.
AU - Sjöblom, P.
AU - Lindenberg, S.
AU - Kimber, S. J.
PY - 1992/6/1
Y1 - 1992/6/1
N2 - The distribution of four cell surface antigens (SSEA-1, SSEA-3, SSEA-4, and I) present on mouse preimplantation embryos was examined on 8-cell stage embryos immediately after flushing from the reproductive tract and after slow or ultra-rapid freezing. Frozen-thawed and nonfrozen embryos were also examined after culture in vitro for 5, 24, or 48 h. Immediately after thawing, embryos showed a disruption in the polarity of cell surface antigens SSEA-3, SSEA-4, and I, but no differences were detected in fluorescence intensity or various other staining characteristics. No long-term changes in the distribution of cell surface components were detected. Implantation and embryonic development were similar for frozen and nonfrozen embryos transferred to the uterine horns of pseudopregnant recipients.
AB - The distribution of four cell surface antigens (SSEA-1, SSEA-3, SSEA-4, and I) present on mouse preimplantation embryos was examined on 8-cell stage embryos immediately after flushing from the reproductive tract and after slow or ultra-rapid freezing. Frozen-thawed and nonfrozen embryos were also examined after culture in vitro for 5, 24, or 48 h. Immediately after thawing, embryos showed a disruption in the polarity of cell surface antigens SSEA-3, SSEA-4, and I, but no differences were detected in fluorescence intensity or various other staining characteristics. No long-term changes in the distribution of cell surface components were detected. Implantation and embryonic development were similar for frozen and nonfrozen embryos transferred to the uterine horns of pseudopregnant recipients.
U2 - 10.1002/jez.1402620313
DO - 10.1002/jez.1402620313
M3 - Article
C2 - 1640203
VL - 262
SP - 330
EP - 339
JO - Journal of Experimental Zoology. Part A: Ecological Genetics and Physiology
JF - Journal of Experimental Zoology. Part A: Ecological Genetics and Physiology
IS - 3
ER -