Effect of solvents on obligately anaerobic bacteria

Maria Fernanda Rodriguez Martinez, Niki Kelessidou, Zoe Law, John Gardiner, Gill Stephens

    Research output: Contribution to journalArticlepeer-review


    Growth of Acetobacterium woodii and Clostridium sporogenes was studied in the presence of water-immiscible solvents. Nitrogen purging, vacuum distillation or distillation under nitrogen were all suitable as methods to remove oxygen from the solvents, since growth rates and yields of A. woodii were unaffected in the presence of tetradecane which had been degassed by these methods. Varying the solvent volume from 20% to 80% of the culture volume had little effect on growth rate of A. woodii. A.woodii was relatively sensitive to organic solvents since growth was inhibited by alkanes with log Poctanol/water values below 7.1. C. sporogenes was less solvent sensitive, since it grew without inhibition when the log P of the solvent was ≥6.6. Nevertheless, both A. woodii and C. sporogenes were more sensitive to solvent polarity than aerobic bacteria. © 2007 Elsevier Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)55-60
    Number of pages5
    Issue number1
    Publication statusPublished - Feb 2008


    • Acetobacterium woodii
    • Clostridium sporogenes
    • Solvent toxicity
    • Two-liquid phase culture systems


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