Effects of estrogen on skeletal myoblast growth

Stefan Kahlert; Christian Grohé; Richard H. Karas; Kerstin Löbbert; Ludwig Neyses; Hans Vetter

doi:10.1006/bbrc.1997.6223

Effects of estrogen on skeletal myoblast growth

Stefan Kahlert, Christian Grohé, Richard H. Karas, Kerstin Löbbert, Ludwig Neyses, Hans Vetter

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Abstract

To determine the role of estrogen in skeletal muscle growth, we investigated estrogen receptor-mediated effects on proliferation in skeletal myoblasts. In L6, C2C12 and Sol8 myoblasts estrogen receptor was demonstrated by immunoblotting, immunofluorescence microscopy and transfection studies. Estrone induced a significant increase in myoblast growth whereas 17β-estradiol had no effect. Furthermore in L6-cells estrone (c-fos: 3.9-fold, egr-1: 4.6-fold) induced immediate-early gene induction significantly stronger than 17β-estradiol (c-fos: 1.7-fold, egr-1: 2.3-fold; p <0.05). Skeletal myoblasts express functional estrogen receptors. Estrogens differ in the activation of skeletal myoblast growth and immediate-early gene induction.

Original language	English
Pages (from-to)	373-378
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	232
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1997.6223
Publication status	Published - 17 Mar 1997

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title = "Effects of estrogen on skeletal myoblast growth",

abstract = "To determine the role of estrogen in skeletal muscle growth, we investigated estrogen receptor-mediated effects on proliferation in skeletal myoblasts. In L6, C2C12 and Sol8 myoblasts estrogen receptor was demonstrated by immunoblotting, immunofluorescence microscopy and transfection studies. Estrone induced a significant increase in myoblast growth whereas 17β-estradiol had no effect. Furthermore in L6-cells estrone (c-fos: 3.9-fold, egr-1: 4.6-fold) induced immediate-early gene induction significantly stronger than 17β-estradiol (c-fos: 1.7-fold, egr-1: 2.3-fold; p <0.05). Skeletal myoblasts express functional estrogen receptors. Estrogens differ in the activation of skeletal myoblast growth and immediate-early gene induction.",

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T1 - Effects of estrogen on skeletal myoblast growth

AU - Kahlert, Stefan

AU - Grohé, Christian

AU - Karas, Richard H.

AU - Löbbert, Kerstin

AU - Neyses, Ludwig

AU - Vetter, Hans

PY - 1997/3/17

Y1 - 1997/3/17

N2 - To determine the role of estrogen in skeletal muscle growth, we investigated estrogen receptor-mediated effects on proliferation in skeletal myoblasts. In L6, C2C12 and Sol8 myoblasts estrogen receptor was demonstrated by immunoblotting, immunofluorescence microscopy and transfection studies. Estrone induced a significant increase in myoblast growth whereas 17β-estradiol had no effect. Furthermore in L6-cells estrone (c-fos: 3.9-fold, egr-1: 4.6-fold) induced immediate-early gene induction significantly stronger than 17β-estradiol (c-fos: 1.7-fold, egr-1: 2.3-fold; p <0.05). Skeletal myoblasts express functional estrogen receptors. Estrogens differ in the activation of skeletal myoblast growth and immediate-early gene induction.

AB - To determine the role of estrogen in skeletal muscle growth, we investigated estrogen receptor-mediated effects on proliferation in skeletal myoblasts. In L6, C2C12 and Sol8 myoblasts estrogen receptor was demonstrated by immunoblotting, immunofluorescence microscopy and transfection studies. Estrone induced a significant increase in myoblast growth whereas 17β-estradiol had no effect. Furthermore in L6-cells estrone (c-fos: 3.9-fold, egr-1: 4.6-fold) induced immediate-early gene induction significantly stronger than 17β-estradiol (c-fos: 1.7-fold, egr-1: 2.3-fold; p <0.05). Skeletal myoblasts express functional estrogen receptors. Estrogens differ in the activation of skeletal myoblast growth and immediate-early gene induction.

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DO - 10.1006/bbrc.1997.6223

M3 - Article

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VL - 232

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EP - 378

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Effects of estrogen on skeletal myoblast growth

Abstract

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