Effects of gentamicin on the proteomes of aerobic and oxygen-limited escherichia coli

Zubida M. Al-Majdoub; Abiola Owoseni; Simon J. Gaskell; Jill Barber

doi:10.1021/jm301858u

Effects of gentamicin on the proteomes of aerobic and oxygen-limited escherichia coli

Zubida M. Al-Majdoub, Abiola Owoseni, Simon J. Gaskell, Jill Barber

Pharmacy

Research output: Contribution to journal › Article › peer-review

Abstract

The key role of the bacterial ribosome makes it an important target for antibacterial agents. Indeed, a large number of clinically useful antibiotics target this complex translational ribonucleoprotein machinery. Unfortunately, the development of resistant bacterial strains has compromised the effectiveness of most classes of antibacterial agent, including the classes that target the ribosome. Combinations of two or more drugs can be used to help overcome resistance, and in certain circumstances their action may be synergistic. In this study we have used proteomic techniques to establish the effects of gentamicin on the proteomes of aerobic and oxygen-limited Escherichia coli. Ribosomal proteins L1, L9, L10, and S2 were found to be up-regulated in both conditions, and we postulate that these are candidate drug targets for the development of synergistic combinations with gentamicin. © 2013 American Chemical Society.

Original language	English
Pages (from-to)	2904-2910
Number of pages	6
Journal	Journal of Medicinal Chemistry
Volume	56
Issue number	7
DOIs	https://doi.org/10.1021/jm301858u
Publication status	Published - 11 Apr 2013

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title = "Effects of gentamicin on the proteomes of aerobic and oxygen-limited escherichia coli",

abstract = "The key role of the bacterial ribosome makes it an important target for antibacterial agents. Indeed, a large number of clinically useful antibiotics target this complex translational ribonucleoprotein machinery. Unfortunately, the development of resistant bacterial strains has compromised the effectiveness of most classes of antibacterial agent, including the classes that target the ribosome. Combinations of two or more drugs can be used to help overcome resistance, and in certain circumstances their action may be synergistic. In this study we have used proteomic techniques to establish the effects of gentamicin on the proteomes of aerobic and oxygen-limited Escherichia coli. Ribosomal proteins L1, L9, L10, and S2 were found to be up-regulated in both conditions, and we postulate that these are candidate drug targets for the development of synergistic combinations with gentamicin. {\textcopyright} 2013 American Chemical Society.",

author = "Al-Majdoub, {Zubida M.} and Abiola Owoseni and Gaskell, {Simon J.} and Jill Barber",

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AU - Al-Majdoub, Zubida M.

AU - Owoseni, Abiola

AU - Gaskell, Simon J.

AU - Barber, Jill

PY - 2013/4/11

Y1 - 2013/4/11

N2 - The key role of the bacterial ribosome makes it an important target for antibacterial agents. Indeed, a large number of clinically useful antibiotics target this complex translational ribonucleoprotein machinery. Unfortunately, the development of resistant bacterial strains has compromised the effectiveness of most classes of antibacterial agent, including the classes that target the ribosome. Combinations of two or more drugs can be used to help overcome resistance, and in certain circumstances their action may be synergistic. In this study we have used proteomic techniques to establish the effects of gentamicin on the proteomes of aerobic and oxygen-limited Escherichia coli. Ribosomal proteins L1, L9, L10, and S2 were found to be up-regulated in both conditions, and we postulate that these are candidate drug targets for the development of synergistic combinations with gentamicin. © 2013 American Chemical Society.

AB - The key role of the bacterial ribosome makes it an important target for antibacterial agents. Indeed, a large number of clinically useful antibiotics target this complex translational ribonucleoprotein machinery. Unfortunately, the development of resistant bacterial strains has compromised the effectiveness of most classes of antibacterial agent, including the classes that target the ribosome. Combinations of two or more drugs can be used to help overcome resistance, and in certain circumstances their action may be synergistic. In this study we have used proteomic techniques to establish the effects of gentamicin on the proteomes of aerobic and oxygen-limited Escherichia coli. Ribosomal proteins L1, L9, L10, and S2 were found to be up-regulated in both conditions, and we postulate that these are candidate drug targets for the development of synergistic combinations with gentamicin. © 2013 American Chemical Society.

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JO - Journal of Medicinal Chemistry

JF - Journal of Medicinal Chemistry

IS - 7

ER -

Effects of gentamicin on the proteomes of aerobic and oxygen-limited escherichia coli

Abstract

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