Effects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implicatioEffects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implications for biological applicationsns for biological applications

Kostas Kostarelos; Chen-Xia Hu; Oliver Read; Yuyoung Shin; Yingxian Chen; Jingjing Wang; Matthew Boyes; Niting Zeng; Adyasha Panigrahi; Igor Larrosa; Sandra Vranic; Cinzia Casiraghi

doi:10.1021/acsanm.2c02403

Effects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implicatioEffects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implications for biological applicationsns for biological applications

Kostas Kostarelos, Chen-Xia Hu, Oliver Read, Yuyoung Shin, Yingxian Chen, Jingjing Wang, Matthew Boyes, Niting Zeng, Adyasha Panigrahi, Igor Larrosa, Sandra Vranic, Cinzia Casiraghi

Research output: Contribution to journal › Article › peer-review

Abstract

In this work, we apply liquid cascade centrifugation to highly concentrated graphene dispersions produced by liquid-phase exfoliation in water with an insoluble bis-pyrene stabilizer to obtain fractions containing nanosheets with different lateral size distributions. The concentration, stability, size, thickness, and the cytotoxicity profile are studied as a function of the initial stabilizer concentration for each fraction. Our results show that there is a critical initial amount of stabilizer (0.4 mg/mL) above which the dispersions show reduced concentration, stability, and biocompatibility, no matter the lateral size of the flakes.

Original language	English
Pages (from-to)	12626–12636
Journal	ACS Applied Nano Materials
Volume	5
Issue number	9
Early online date	12 Sept 2022
DOIs	https://doi.org/10.1021/acsanm.2c02403
Publication status	Published - 23 Sept 2022

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Kostarelos, K., Hu, C.-X., Read, O., Shin, Y., Chen, Y., Wang, J., Boyes, M., Zeng, N., Panigrahi, A., Larrosa, I., Vranic, S., & Casiraghi, C. (2022). Effects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implicatioEffects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implications for biological applicationsns for biological applications. ACS Applied Nano Materials, 5(9), 12626–12636. https://doi.org/10.1021/acsanm.2c02403

Kostarelos, Kostas ; Hu, Chen-Xia ; Read, Oliver et al. / Effects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implicatioEffects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implications for biological applicationsns for biological applications. In: ACS Applied Nano Materials. 2022 ; Vol. 5, No. 9. pp. 12626–12636.

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title = "Effects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implicatioEffects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implications for biological applicationsns for biological applications",

abstract = "In this work, we apply liquid cascade centrifugation to highly concentrated graphene dispersions produced by liquid-phase exfoliation in water with an insoluble bis-pyrene stabilizer to obtain fractions containing nanosheets with different lateral size distributions. The concentration, stability, size, thickness, and the cytotoxicity profile are studied as a function of the initial stabilizer concentration for each fraction. Our results show that there is a critical initial amount of stabilizer (0.4 mg/mL) above which the dispersions show reduced concentration, stability, and biocompatibility, no matter the lateral size of the flakes.",

author = "Kostas Kostarelos and Chen-Xia Hu and Oliver Read and Yuyoung Shin and Yingxian Chen and Jingjing Wang and Matthew Boyes and Niting Zeng and Adyasha Panigrahi and Igor Larrosa and Sandra Vranic and Cinzia Casiraghi",

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doi = "10.1021/acsanm.2c02403",

language = "English",

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Kostarelos, K, Hu, C-X, Read, O, Shin, Y, Chen, Y, Wang, J, Boyes, M, Zeng, N, Panigrahi, A, Larrosa, I , Vranic, S & Casiraghi, C 2022, 'Effects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implicatioEffects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implications for biological applicationsns for biological applications', ACS Applied Nano Materials, vol. 5, no. 9, pp. 12626–12636. https://doi.org/10.1021/acsanm.2c02403

Effects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implicatioEffects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implications for biological applicationsns for biological applications. / Kostarelos, Kostas; Hu, Chen-Xia; Read, Oliver et al.
In: ACS Applied Nano Materials, Vol. 5, No. 9, 23.09.2022, p. 12626–12636.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Effects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implicatioEffects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implications for biological applicationsns for biological applications

AU - Kostarelos, Kostas

AU - Hu, Chen-Xia

AU - Read, Oliver

AU - Shin, Yuyoung

AU - Chen, Yingxian

AU - Wang, Jingjing

AU - Boyes, Matthew

AU - Zeng, Niting

AU - Panigrahi, Adyasha

AU - Larrosa, Igor

AU - Vranic, Sandra

AU - Casiraghi, Cinzia

PY - 2022/9/23

Y1 - 2022/9/23

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AB - In this work, we apply liquid cascade centrifugation to highly concentrated graphene dispersions produced by liquid-phase exfoliation in water with an insoluble bis-pyrene stabilizer to obtain fractions containing nanosheets with different lateral size distributions. The concentration, stability, size, thickness, and the cytotoxicity profile are studied as a function of the initial stabilizer concentration for each fraction. Our results show that there is a critical initial amount of stabilizer (0.4 mg/mL) above which the dispersions show reduced concentration, stability, and biocompatibility, no matter the lateral size of the flakes.

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DO - 10.1021/acsanm.2c02403

M3 - Article

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JO - ACS Applied Nano Materials

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Kostarelos K, Hu CX, Read O, Shin Y, Chen Y, Wang J et al. Effects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implicatioEffects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implications for biological applicationsns for biological applications. ACS Applied Nano Materials. 2022 Sept 23;5(9):12626–12636. Epub 2022 Sept 12. doi: 10.1021/acsanm.2c02403