Efficient downregulation of alb1 gene using an AMA1-based episomal expression of RNAi construct in Aspergillus fumigatus

Vahid Khalaj; Hamid Eslami; Mohammad Azizi; Nuria Rovira-Graells; Mike Bromley

doi:10.1111/j.1574-6968.2007.00680.x

Efficient downregulation of alb1 gene using an AMA1-based episomal expression of RNAi construct in Aspergillus fumigatus

Vahid Khalaj, Hamid Eslami, Mohammad Azizi, Nuria Rovira-Graells, Mike Bromley

Research output: Contribution to journal › Article › peer-review

Abstract

An episomal RNAi silencing construct containing the inducible cbhB promoter and a hairpin structure has been made to downregulate the alb1 gene in the human pathogen Aspergillus fumigatus. Transformation of fungal protoplasts resulted in a high number of transformants with an inducible silenced phenotype (white spores). Efficient downregulation of the alb1 gene using this system suggests that this approach may overcome the variable downregulation observed with integrative constructs. © 2007 Federation of European Microbiological Societies.

Original language	English
Pages (from-to)	250-254
Number of pages	4
Journal	FEMS microbiology letters
Volume	270
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.2007.00680.x
Publication status	Published - May 2007

Keywords

AMA1
Aspergillus
cbhB
RNAi

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10.1111/j.1574-6968.2007.00680.x

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abstract = "An episomal RNAi silencing construct containing the inducible cbhB promoter and a hairpin structure has been made to downregulate the alb1 gene in the human pathogen Aspergillus fumigatus. Transformation of fungal protoplasts resulted in a high number of transformants with an inducible silenced phenotype (white spores). Efficient downregulation of the alb1 gene using this system suggests that this approach may overcome the variable downregulation observed with integrative constructs. {\textcopyright} 2007 Federation of European Microbiological Societies.",

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author = "Vahid Khalaj and Hamid Eslami and Mohammad Azizi and Nuria Rovira-Graells and Mike Bromley",

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AU - Khalaj, Vahid

AU - Eslami, Hamid

AU - Azizi, Mohammad

AU - Rovira-Graells, Nuria

AU - Bromley, Mike

PY - 2007/5

Y1 - 2007/5

N2 - An episomal RNAi silencing construct containing the inducible cbhB promoter and a hairpin structure has been made to downregulate the alb1 gene in the human pathogen Aspergillus fumigatus. Transformation of fungal protoplasts resulted in a high number of transformants with an inducible silenced phenotype (white spores). Efficient downregulation of the alb1 gene using this system suggests that this approach may overcome the variable downregulation observed with integrative constructs. © 2007 Federation of European Microbiological Societies.

AB - An episomal RNAi silencing construct containing the inducible cbhB promoter and a hairpin structure has been made to downregulate the alb1 gene in the human pathogen Aspergillus fumigatus. Transformation of fungal protoplasts resulted in a high number of transformants with an inducible silenced phenotype (white spores). Efficient downregulation of the alb1 gene using this system suggests that this approach may overcome the variable downregulation observed with integrative constructs. © 2007 Federation of European Microbiological Societies.

KW - AMA1

KW - Aspergillus

KW - cbhB

KW - RNAi

U2 - 10.1111/j.1574-6968.2007.00680.x

DO - 10.1111/j.1574-6968.2007.00680.x

M3 - Article

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SN - 1574-6968

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JF - FEMS microbiology letters

IS - 2

ER -

Efficient downregulation of alb1 gene using an AMA1-based episomal expression of RNAi construct in Aspergillus fumigatus

Abstract

Keywords

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