Efficient mediator-independent hydrogenation of carbon-carbon double bonds, using the obligate anaerobe, Acetobacterium woodii, with fructose as an electron donor

E. T. Davies, G. M. Stephens

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Fructose and H 2 were compared as electron donors for hydrogenation of carbon-carbon double bonds using Acetobacter woodii. Caffeate was used as a model substrate. An electron donor was required and both fructose and H 2 were suitable. With fructose as the donor, the K(s) for caffeate was 0.5 mM and the V(max) was 678 mmol kg(dry weight) -1 h -1. Fructose oxidation was coupled very efficiently to caffeate reduction by an alteration in the fructose fermentation so that acetate was no longer produced.
    Original languageEnglish
    Pages (from-to)615-618
    Number of pages3
    JournalApplied microbiology and biotechnology
    Volume46
    Issue number5-6
    Publication statusPublished - 1996

    Keywords

    • Acetobacterium woodii
    • Double bond
    • Electron donors
    • Hydrogenation (mediator-independent hydrogenation of carbon-carbon double bonds with Acetobacterium woodii and fructose and H2 as electron donors)
    • Acetobacter hydrogenation fructose

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