Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma

Ivona Baricevic-Jones, Milan Nikolić, Dragana Stanić, Ivona Baričević, David R. Jones, Olgica Nedić, Vesna Niketić

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Objective: The interior of red blood cells (RBCs) contains a variable amount of cholesterol and phospholipids bound to haemoglobin (Hb). This current study was devised to determine if this pool of lipids (termed Hb-Ch) was available for exchange with plasma lipoproteins. Design and methods: We studied the in vitro efflux of lipids from human RBCs into fasting plasma in men with either low (control group) or high Hb-Ch (study group). Results: When plasma was incubated with a two-fold excess of autologous RBCs the plasma cholesterol level increased due to a decrease in the level of cholesterol from the RBC membrane (in the control group) and due to a decrease in the level of cholesterol both from the RBC membrane and the Hb-Ch fraction (in the study group). The loss of Hb-Ch-derived phospholipids during lipid efflux was roughly equal to that of Hb-Ch-derived cholesterol. The loss of RBC cholesterol into plasma high-density lipoproteins (HDL) was more pronounced in our study group and correlated with the loss of cholesterol from Hb-Ch. Conclusion: The Hb-Ch adduct significantly contributes to the lipid efflux from RBCs into plasma. The majority of cholesterol released from Hb-Ch appears in the plasma HDL fraction suggesting that Hb-Ch may play a role in reverse cholesterol transport in vivo. © 2006 The Canadian Society of Clinical Chemists.
    Original languageEnglish
    Pages (from-to)305-309
    Number of pages4
    JournalClinical biochemistry
    Volume40
    Issue number5-6
    DOIs
    Publication statusPublished - Mar 2007

    Keywords

    • Cholesterol
    • Haemoglobin
    • HDL cholesterol
    • Lipoproteins
    • Phospholipids
    • Red blood cells

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