Endothelial cell activation by interleukin-1 and extracellular matrix laminin-10 occurs via the YAP signalling pathway

Hannah Thurgur; Jeffrey Penny; Emmanuel Pinteaux

doi:10.1016/j.jneuroim.2022.577993

Endothelial cell activation by interleukin-1 and extracellular matrix laminin-10 occurs via the YAP signalling pathway

Hannah Thurgur, Jeffrey Penny, Emmanuel Pinteaux

Research output: Contribution to journal › Article › peer-review

Abstract

Laminin-10 (LM-10) is a key regulator of blood-brain barrier (BBB) repair after hypoxia and inflammation. Here we investigated the signalling mechanisms regulated by LM-10 in human brain endothelial cell line hCMEC/D3 in response to interleukin(IL)-1beta(β) in vitro. LM-10 promoted endothelial proliferation and repair of an endothelial monolayer after scratch injury, and upregulated IL-1β-induced ICAM-1 and VCAM-1 expression. IL-1β and LM-10 regulated YAP signalling pathway in endothelial cells leading to differential expression of YAP target genes, ctgf and serpine-1, providing evidence that the YAP signalling pathway could be a new therapeutic target for the treatment of BBB dysfunction in CNS diseases.

Original language	English
Article number	577993
Journal	J Neuroimmunology,
Volume	373
Early online date	28 Oct 2022
DOIs	https://doi.org/10.1016/j.jneuroim.2022.577993
Publication status	Published - 15 Dec 2022

Keywords

Angiogenesis
Brain endothelia
Extracellular matrix
Inflammation
Interleukin
Laminin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jneuroim.2022.577993Licence: CC BY

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title = "Endothelial cell activation by interleukin-1 and extracellular matrix laminin-10 occurs via the YAP signalling pathway",

abstract = "Laminin-10 (LM-10) is a key regulator of blood-brain barrier (BBB) repair after hypoxia and inflammation. Here we investigated the signalling mechanisms regulated by LM-10 in human brain endothelial cell line hCMEC/D3 in response to interleukin(IL)-1beta(β) in vitro. LM-10 promoted endothelial proliferation and repair of an endothelial monolayer after scratch injury, and upregulated IL-1β-induced ICAM-1 and VCAM-1 expression. IL-1β and LM-10 regulated YAP signalling pathway in endothelial cells leading to differential expression of YAP target genes, ctgf and serpine-1, providing evidence that the YAP signalling pathway could be a new therapeutic target for the treatment of BBB dysfunction in CNS diseases.",

keywords = "Angiogenesis, Brain endothelia, Extracellular matrix, Inflammation, Interleukin, Laminin",

author = "Hannah Thurgur and Jeffrey Penny and Emmanuel Pinteaux",

year = "2022",

month = dec,

day = "15",

doi = "10.1016/j.jneuroim.2022.577993",

language = "English",

volume = "373",

journal = "J Neuroimmunology,",

issn = "0165-5728",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Endothelial cell activation by interleukin-1 and extracellular matrix laminin-10 occurs via the YAP signalling pathway

AU - Thurgur, Hannah

AU - Penny, Jeffrey

AU - Pinteaux, Emmanuel

PY - 2022/12/15

Y1 - 2022/12/15

N2 - Laminin-10 (LM-10) is a key regulator of blood-brain barrier (BBB) repair after hypoxia and inflammation. Here we investigated the signalling mechanisms regulated by LM-10 in human brain endothelial cell line hCMEC/D3 in response to interleukin(IL)-1beta(β) in vitro. LM-10 promoted endothelial proliferation and repair of an endothelial monolayer after scratch injury, and upregulated IL-1β-induced ICAM-1 and VCAM-1 expression. IL-1β and LM-10 regulated YAP signalling pathway in endothelial cells leading to differential expression of YAP target genes, ctgf and serpine-1, providing evidence that the YAP signalling pathway could be a new therapeutic target for the treatment of BBB dysfunction in CNS diseases.

AB - Laminin-10 (LM-10) is a key regulator of blood-brain barrier (BBB) repair after hypoxia and inflammation. Here we investigated the signalling mechanisms regulated by LM-10 in human brain endothelial cell line hCMEC/D3 in response to interleukin(IL)-1beta(β) in vitro. LM-10 promoted endothelial proliferation and repair of an endothelial monolayer after scratch injury, and upregulated IL-1β-induced ICAM-1 and VCAM-1 expression. IL-1β and LM-10 regulated YAP signalling pathway in endothelial cells leading to differential expression of YAP target genes, ctgf and serpine-1, providing evidence that the YAP signalling pathway could be a new therapeutic target for the treatment of BBB dysfunction in CNS diseases.

KW - Angiogenesis

KW - Brain endothelia

KW - Extracellular matrix

KW - Inflammation

KW - Interleukin

KW - Laminin

U2 - 10.1016/j.jneuroim.2022.577993

DO - 10.1016/j.jneuroim.2022.577993

M3 - Article

SN - 0165-5728

VL - 373

JO - J Neuroimmunology,

JF - J Neuroimmunology,

M1 - 577993

ER -

Endothelial cell activation by interleukin-1 and extracellular matrix laminin-10 occurs via the YAP signalling pathway

Abstract

Keywords

UN SDGs

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