Enhanced FTIR bench-top imaging of single biological cells

Caryn Hughes; Alex Henderson; M Kaniz; Konrad Dorling; Melody Jimenez Hernandez; Mick Brown; Noel Clarke; Peter Gardner

doi:10.1039/C4AN02053G

Enhanced FTIR bench-top imaging of single biological cells

Caryn Hughes, Alex Henderson, M Kaniz, Konrad Dorling, Melody Jimenez Hernandez, Mick Brown, Noel Clarke, Peter Gardner

Research output: Contribution to journal › Article › peer-review

Abstract

A new optical system has recently been developed that enables infrared images to be obtained with a pixel resolution of 1 micron on a bench-top instrument using a thermal source. We present here imaging data from two contrasting cellular systems that represent different challenges. Renal carcinoma cells cytospun onto CaF2 have a largely rounded morphology and thus suffer from strong resonant Mie scattering. Skin fibroblast cells, cultured onto CaF2 on the other hand are very spread out so scatter less strongly but are so thin they deliver extremely weak signals. Using suitable pre-processing methods, including PCA noise reduction and RMieS correction, we demonstrate that useful high resolution images can be obtained from either sample.

Original language	English
Pages (from-to)	2080-2085
Number of pages	5
Journal	The Analyst
Volume	140
Issue number	7
Early online date	20 Feb 2015
DOIs	https://doi.org/10.1039/C4AN02053G
Publication status	Published - 7 Apr 2015

Keywords

Infrared, High magnification, Single cell imaging
Rencal Cancer, FTIR

Research Beacons, Institutes and Platforms

Manchester Institute of Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

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title = "Enhanced FTIR bench-top imaging of single biological cells",

abstract = "A new optical system has recently been developed that enables infrared images to be obtained with a pixel resolution of 1 micron on a bench-top instrument using a thermal source. We present here imaging data from two contrasting cellular systems that represent different challenges. Renal carcinoma cells cytospun onto CaF2 have a largely rounded morphology and thus suffer from strong resonant Mie scattering. Skin fibroblast cells, cultured onto CaF2 on the other hand are very spread out so scatter less strongly but are so thin they deliver extremely weak signals. Using suitable pre-processing methods, including PCA noise reduction and RMieS correction, we demonstrate that useful high resolution images can be obtained from either sample.",

keywords = "Infrared, High magnification, Single cell imaging, Rencal Cancer, FTIR",

author = "Caryn Hughes and Alex Henderson and M Kaniz and Konrad Dorling and {Jimenez Hernandez}, Melody and Mick Brown and Noel Clarke and Peter Gardner",

note = "This work was funded by the UK{\textquoteright}s Engineering and Physical Sciences Research Council on grant number EP/I027440/1.",

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doi = "10.1039/C4AN02053G",

language = "English",

volume = "140",

pages = "2080--2085",

journal = "The Analyst",

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TY - JOUR

T1 - Enhanced FTIR bench-top imaging of single biological cells

AU - Hughes, Caryn

AU - Henderson, Alex

AU - Kaniz, M

AU - Dorling, Konrad

AU - Jimenez Hernandez, Melody

AU - Brown, Mick

AU - Clarke, Noel

AU - Gardner, Peter

N1 - This work was funded by the UK’s Engineering and Physical Sciences Research Council on grant number EP/I027440/1.

PY - 2015/4/7

Y1 - 2015/4/7

N2 - A new optical system has recently been developed that enables infrared images to be obtained with a pixel resolution of 1 micron on a bench-top instrument using a thermal source. We present here imaging data from two contrasting cellular systems that represent different challenges. Renal carcinoma cells cytospun onto CaF2 have a largely rounded morphology and thus suffer from strong resonant Mie scattering. Skin fibroblast cells, cultured onto CaF2 on the other hand are very spread out so scatter less strongly but are so thin they deliver extremely weak signals. Using suitable pre-processing methods, including PCA noise reduction and RMieS correction, we demonstrate that useful high resolution images can be obtained from either sample.

AB - A new optical system has recently been developed that enables infrared images to be obtained with a pixel resolution of 1 micron on a bench-top instrument using a thermal source. We present here imaging data from two contrasting cellular systems that represent different challenges. Renal carcinoma cells cytospun onto CaF2 have a largely rounded morphology and thus suffer from strong resonant Mie scattering. Skin fibroblast cells, cultured onto CaF2 on the other hand are very spread out so scatter less strongly but are so thin they deliver extremely weak signals. Using suitable pre-processing methods, including PCA noise reduction and RMieS correction, we demonstrate that useful high resolution images can be obtained from either sample.

KW - Infrared, High magnification, Single cell imaging

KW - Rencal Cancer, FTIR

U2 - 10.1039/C4AN02053G

DO - 10.1039/C4AN02053G

M3 - Article

SN - 0003-2654

VL - 140

SP - 2080

EP - 2085

JO - The Analyst

JF - The Analyst

IS - 7

ER -

Enhanced FTIR bench-top imaging of single biological cells

Abstract

Keywords

Research Beacons, Institutes and Platforms

UN SDGs

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