Enhanced FTIR bench-top imaging of single biological cells

Caryn Hughes, Alex Henderson, M Kaniz, Konrad Dorling, Melody Jimenez Hernandez, Mick Brown, Noel Clarke, Peter Gardner

    Research output: Contribution to journalArticlepeer-review


    A new optical system has recently been developed that enables infrared images to be obtained with a pixel resolution of 1 micron on a bench-top instrument using a thermal source. We present here imaging data from two contrasting cellular systems that represent different challenges. Renal carcinoma cells cytospun onto CaF2 have a largely rounded morphology and thus suffer from strong resonant Mie scattering. Skin fibroblast cells, cultured onto CaF2 on the other hand are very spread out so scatter less strongly but are so thin they deliver extremely weak signals. Using suitable pre-processing methods, including PCA noise reduction and RMieS correction, we demonstrate that useful high resolution images can be obtained from either sample.
    Original languageEnglish
    Pages (from-to)2080-2085
    Number of pages5
    JournalThe Analyst
    Issue number7
    Early online date20 Feb 2015
    Publication statusPublished - 7 Apr 2015


    • Infrared, High magnification, Single cell imaging
    • Rencal Cancer, FTIR

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology


    Dive into the research topics of 'Enhanced FTIR bench-top imaging of single biological cells'. Together they form a unique fingerprint.

    Cite this