Enhanced phagocytosis and intracellular killing of Candida albicans by GM-CSF-activated human neutrophils.

M D Richardson, C E Brownlie, G S Shankland

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Little is known about effector cell modulation regarding phagocytosis and candidacidal activities of human neutrophils in response to granulocyte-macrophage colony stimulating factor (GM-CSF). In neutrophil monolayer assays, pre-treatment of cells with ultrapure GM-CSF at 40 Units ml-1 for a minimum of 1 h showed a significant enhancement of phagocytosis of Candida albicans (phagocytic index of 2.6 compared with 1.6 for unactivated neutrophils) after a 20 min ingestion period (P <0.001). By counting viable colonies 50% of intracellular yeast cells were killed by non-activated neutrophils following internalization for 2 h. Neutrophils activated for 2 h with 40 Units ml-1 of GM-CSF killed 90% of intracellular blastoconidia (P <0.02). These results demonstrate that in in vitro assays GM-CSF enhances phagocytosis and intracellular killing of C. albicans.
    Original languageEnglish
    JournalJournal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology
    Volume30
    Issue number6
    Publication statusPublished - 1992

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