TY - JOUR
T1 - Environmental and genetic determinants of vitamin D insufficiency in 12-month-old infants.
AU - Suaini, Noor H A
AU - Koplin, Jennifer J
AU - Ellis, Justine A
AU - Peters, Rachel L
AU - Ponsonby, Anne-Louise
AU - Dharmage, Shyamali C
AU - Matheson, Melanie C
AU - Wake, Melissa
AU - Panjari, Mary
AU - Tan, Hern-Tze Tina
AU - Martin, Pamela E
AU - Pezic, Angela
AU - Lowe, Adrian J
AU - Martino, David
AU - Gurrin, Lyle C
AU - Vuillermin, Peter J
AU - Tang, Mimi L K
AU - Allen, Katrina J
A2 - Thiele, Leone
A2 - Czech, Helen
A2 - Hill, David
A2 - Anderson, Deborah
A2 - Eckert, Jana
A2 - Sanjeevan, Jeeva
A2 - Dwyer, Terry
A2 - Dang, Thanh
A2 - Osborne, Nicholas
A2 - Robinson, Marnie
A2 - Zurzolo, Dean Tey Giovanni
A2 - Bertalli, Nadine
A2 - Cranswick, Noel
A2 - Smart, Jo
A2 - Douglass, Jo
PY - 2014/10
Y1 - 2014/10
N2 - We aimed to investigate the relationship between genetic and environmental exposure and vitamin D status at age one, stratified by ethnicity. This study included 563 12-month-old infants in the HealthNuts population-based study. DNA from participants' blood samples was genotyped using Sequenom MassARRAY MALDI-TOF system on 28 single nucleotide polymorphisms (SNPs) in six genes. Using logistic regression, we examined associations between environmental exposure and SNPs in vitamin D pathway and filaggrin genes and vitamin D insufficiency (VDI). VDI, defined as serum 25-hydroxyvitamin D3(25(OH)D3) level ≤50nmol/L, was measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Infants were stratified by ethnicity determined by parent's country of birth. Infants formula fed at 12 months were associated with reduced odds of VDI compared to infants with no current formula use at 12 months. This association differed by ethnicity (Pinteraction=0.01). The odds ratio (OR) of VDI was 0.29 for Caucasian infants (95% CI, 0.18-0.47) and 0.04 for Asian infants (95% CI, 0.006-0.23). Maternal vitamin D supplementation during pregnancy and/or breastfeeding were associated with increased odds of infants being VDI (OR, 2.39; 95% CI, 1.11-5.18 and OR, 2.5; 95% CI, 1.20-5.24 respectively). Presence of a minor allele for any GC SNP (rs17467825, rs1155563, rs2282679, rs3755967, rs4588, rs7041) was associated with increased odds of VDI. Caucasian infants homozygous (AA) for rs4588 had an OR of 2.49 of being associated with VDI (95% CI, 1.19-5.18). In a country without routine infant vitamin D supplementation or food chain fortification, formula use is strongly associated with a reduced risk of VDI regardless of ethnicity. There was borderline significance for an association between filaggrin mutations and VDI. However, polymorphisms in vitamin D pathway related genes were associated with increased likelihood of being VDI in infancy.
AB - We aimed to investigate the relationship between genetic and environmental exposure and vitamin D status at age one, stratified by ethnicity. This study included 563 12-month-old infants in the HealthNuts population-based study. DNA from participants' blood samples was genotyped using Sequenom MassARRAY MALDI-TOF system on 28 single nucleotide polymorphisms (SNPs) in six genes. Using logistic regression, we examined associations between environmental exposure and SNPs in vitamin D pathway and filaggrin genes and vitamin D insufficiency (VDI). VDI, defined as serum 25-hydroxyvitamin D3(25(OH)D3) level ≤50nmol/L, was measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Infants were stratified by ethnicity determined by parent's country of birth. Infants formula fed at 12 months were associated with reduced odds of VDI compared to infants with no current formula use at 12 months. This association differed by ethnicity (Pinteraction=0.01). The odds ratio (OR) of VDI was 0.29 for Caucasian infants (95% CI, 0.18-0.47) and 0.04 for Asian infants (95% CI, 0.006-0.23). Maternal vitamin D supplementation during pregnancy and/or breastfeeding were associated with increased odds of infants being VDI (OR, 2.39; 95% CI, 1.11-5.18 and OR, 2.5; 95% CI, 1.20-5.24 respectively). Presence of a minor allele for any GC SNP (rs17467825, rs1155563, rs2282679, rs3755967, rs4588, rs7041) was associated with increased odds of VDI. Caucasian infants homozygous (AA) for rs4588 had an OR of 2.49 of being associated with VDI (95% CI, 1.19-5.18). In a country without routine infant vitamin D supplementation or food chain fortification, formula use is strongly associated with a reduced risk of VDI regardless of ethnicity. There was borderline significance for an association between filaggrin mutations and VDI. However, polymorphisms in vitamin D pathway related genes were associated with increased likelihood of being VDI in infancy.
KW - Breastfeeding
KW - Cohort studies
KW - Genetic association studies
KW - Infant formula
KW - SNPs
KW - Vitamin D
U2 - 10.1016/j.jsbmb.2014.08.018
DO - 10.1016/j.jsbmb.2014.08.018
M3 - Article
C2 - 25174667
SN - 0960-0760
VL - 144 Pt B
JO - The Journal of steroid biochemistry and molecular biology
JF - The Journal of steroid biochemistry and molecular biology
ER -