Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis

M. Grattieri; S. Babanova; C. Santoro; E. Guerrini; S.P. Trasatti; P. Cristiani; M. Bestetti; P. Atanassov

doi:10.1002/elan.201400543

Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis

M. Grattieri, S. Babanova, C. Santoro, E. Guerrini, S.P. Trasatti, P. Cristiani, M. Bestetti, P. Atanassov

CE - Academic & Research

Research output: Contribution to journal › Article › peer-review

Abstract

A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single- chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was opti- mized by a diffusion layer of silica gel. A computer-con-
trolled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3 mg Lﰟ1 of O2 in the bulk solution, decreasing to 0 mg Lﰟ1 in the cathodic bio- film. The MFC generated power in the range of 0– 0.08 mW, associated to the oxygen content.

Original language	English
Pages (from-to)	327-335
Number of pages	9
Journal	Electroanalysis
Volume	27
DOIs	https://doi.org/10.1002/elan.201400543
Publication status	Published - 2015

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10.1002/elan.201400543

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title = "Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis",

abstract = "A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single- chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was opti- mized by a diffusion layer of silica gel. A computer-con-trolled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3 mg Lﰟ1 of O2 in the bulk solution, decreasing to 0 mg Lﰟ1 in the cathodic bio- film. The MFC generated power in the range of 0– 0.08 mW, associated to the oxygen content.",

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T1 - Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis

AU - Grattieri, M.

AU - Babanova, S.

AU - Santoro, C.

AU - Guerrini, E.

AU - Trasatti, S.P.

AU - Cristiani, P.

AU - Bestetti, M.

AU - Atanassov, P.

PY - 2015

Y1 - 2015

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AB - A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single- chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was opti- mized by a diffusion layer of silica gel. A computer-con-trolled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3 mg Lﰟ1 of O2 in the bulk solution, decreasing to 0 mg Lﰟ1 in the cathodic bio- film. The MFC generated power in the range of 0– 0.08 mW, associated to the oxygen content.

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DO - 10.1002/elan.201400543

M3 - Article

SN - 1040-0397

VL - 27

SP - 327

EP - 335

JO - Electroanalysis

JF - Electroanalysis

ER -

Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis

Abstract

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