Enzymatic synthesis of colorimetric substrates to determine α-2,3- and α-2,6-specific neuraminidase activity

Juana Elizabeth Reyes Martínez; Robert Šardzík; Josef Voglmeir; Sabine L. Flitsch

doi:10.1039/c3ra44791j

Enzymatic synthesis of colorimetric substrates to determine α-2,3- and α-2,6-specific neuraminidase activity

Juana Elizabeth Reyes Martínez, Robert Šardzík, Josef Voglmeir, Sabine L. Flitsch

Research output: Contribution to journal › Article › peer-review

Abstract

Glycoconjugates containing either terminal α-2,3- or α-2,6-Neu5Ac-Gal disaccharides are found on cell surfaces of many animal glycans. Each linkage can be specifically recognized by lectins and enzymes such as neuraminidases. Here we describe a one-step enzymatic synthesis of two colorimetric substrates that allow for fast distinction of specific neuraminidase activity. This journal is © The Royal Society of Chemistry 2013.

Original language	English
Pages (from-to)	21335-21338
Number of pages	3
Journal	RSC Advances
Volume	3
Issue number	44
DOIs	https://doi.org/10.1039/c3ra44791j
Publication status	Published - 2013

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title = "Enzymatic synthesis of colorimetric substrates to determine α-2,3- and α-2,6-specific neuraminidase activity",

abstract = "Glycoconjugates containing either terminal α-2,3- or α-2,6-Neu5Ac-Gal disaccharides are found on cell surfaces of many animal glycans. Each linkage can be specifically recognized by lectins and enzymes such as neuraminidases. Here we describe a one-step enzymatic synthesis of two colorimetric substrates that allow for fast distinction of specific neuraminidase activity. This journal is {\textcopyright} The Royal Society of Chemistry 2013.",

author = "{Reyes Mart{\'i}nez}, {Juana Elizabeth} and Robert {\v S}ardz{\'i}k and Josef Voglmeir and Flitsch, {Sabine L.}",

year = "2013",

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language = "English",

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T1 - Enzymatic synthesis of colorimetric substrates to determine α-2,3- and α-2,6-specific neuraminidase activity

AU - Reyes Martínez, Juana Elizabeth

AU - Šardzík, Robert

AU - Voglmeir, Josef

AU - Flitsch, Sabine L.

PY - 2013

Y1 - 2013

N2 - Glycoconjugates containing either terminal α-2,3- or α-2,6-Neu5Ac-Gal disaccharides are found on cell surfaces of many animal glycans. Each linkage can be specifically recognized by lectins and enzymes such as neuraminidases. Here we describe a one-step enzymatic synthesis of two colorimetric substrates that allow for fast distinction of specific neuraminidase activity. This journal is © The Royal Society of Chemistry 2013.

AB - Glycoconjugates containing either terminal α-2,3- or α-2,6-Neu5Ac-Gal disaccharides are found on cell surfaces of many animal glycans. Each linkage can be specifically recognized by lectins and enzymes such as neuraminidases. Here we describe a one-step enzymatic synthesis of two colorimetric substrates that allow for fast distinction of specific neuraminidase activity. This journal is © The Royal Society of Chemistry 2013.

U2 - 10.1039/c3ra44791j

DO - 10.1039/c3ra44791j

M3 - Article

SN - 2046-2069

VL - 3

SP - 21335

EP - 21338

JO - RSC Advances

JF - RSC Advances

IS - 44

ER -

Enzymatic synthesis of colorimetric substrates to determine α-2,3- and α-2,6-specific neuraminidase activity

Abstract

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