Enzymatic synthesis of peptides on a solid support

Rose Haddoub; Martin Dauner; Fiona A. Stefanowicz; Valeria Barattini; Nicolas Laurent; Sabine L. Flitsch

doi:10.1039/b816847d

Enzymatic synthesis of peptides on a solid support

Rose Haddoub, Martin Dauner, Fiona A. Stefanowicz, Valeria Barattini, Nicolas Laurent, Sabine L. Flitsch

Research output: Contribution to journal › Article › peer-review

Abstract

We have previously shown that dipeptides can be synthesised in high yields from amino acids using protease catalysis in aqueous media, if the amino component is immobilised on porous PEGA resin (a copolymer of polyethylene glycol and polyacrylamide). Here we explore the scope of this methodology for using protected and glycosylated amino acids as well as the synthesis of longer peptides on resin and show that such a method can also be applied on non-porous surfaces, in particular on gold. © 2009 The Royal Society of Chemistry.

Original language	English
Pages (from-to)	665-670
Number of pages	5
Journal	Organic and Biomolecular Chemistry
Volume	7
Issue number	4
DOIs	https://doi.org/10.1039/b816847d
Publication status	Published - 2009

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title = "Enzymatic synthesis of peptides on a solid support",

abstract = "We have previously shown that dipeptides can be synthesised in high yields from amino acids using protease catalysis in aqueous media, if the amino component is immobilised on porous PEGA resin (a copolymer of polyethylene glycol and polyacrylamide). Here we explore the scope of this methodology for using protected and glycosylated amino acids as well as the synthesis of longer peptides on resin and show that such a method can also be applied on non-porous surfaces, in particular on gold. {\textcopyright} 2009 The Royal Society of Chemistry.",

author = "Rose Haddoub and Martin Dauner and Stefanowicz, {Fiona A.} and Valeria Barattini and Nicolas Laurent and Flitsch, {Sabine L.}",

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AU - Haddoub, Rose

AU - Dauner, Martin

AU - Stefanowicz, Fiona A.

AU - Barattini, Valeria

AU - Laurent, Nicolas

AU - Flitsch, Sabine L.

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AB - We have previously shown that dipeptides can be synthesised in high yields from amino acids using protease catalysis in aqueous media, if the amino component is immobilised on porous PEGA resin (a copolymer of polyethylene glycol and polyacrylamide). Here we explore the scope of this methodology for using protected and glycosylated amino acids as well as the synthesis of longer peptides on resin and show that such a method can also be applied on non-porous surfaces, in particular on gold. © 2009 The Royal Society of Chemistry.

U2 - 10.1039/b816847d

DO - 10.1039/b816847d

M3 - Article

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VL - 7

SP - 665

EP - 670

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

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ER -

Enzymatic synthesis of peptides on a solid support

Abstract

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