TY - JOUR
T1 - Enzymatically-tailored pectins differentially influence the morphology, adhesion, cell cycle progression and survival of fibroblasts
AU - Nagel, Marie Danielle
AU - Verhoef, René
AU - Schols, Henk
AU - Morra, Marco
AU - Knox, J. Paul
AU - Ceccone, Giacomo
AU - Della Volpe, Claudio
AU - Vigneron, Pascale
AU - Bussy, Cyrill
AU - Gallet, Marlène
AU - Velzenberger, Elodie
AU - Vayssade, Muriel
AU - Cascardo, Giovanna
AU - Cassinelli, Clara
AU - Haeger, Ash
AU - Gilliland, Douglas
AU - Liakos, Ioannis
AU - Rodriguez-Valverde, Miguel
AU - Siboni, Stefano
N1 - 321MJ Times Cited:12 Cited References Count:60
PY - 2008/7
Y1 - 2008/7
N2 - Improved biocompatibility and performance of biomedical devices can be achieved through the incorporation of bioactive molecules on device surfaces. Five structurally distinct pectic polysaccharides (modified hairy regions (MHRs)) were obtained by enzymatic liquefaction of apple (MHR-B, MHR-A and MHR-α), carrot (MHR-C) and potato (MHR-P) cells. Polystyrene (PS) Petri dishes, aminated by a plasma deposition process, were surface modified by the covalent linking of the MHRs. Results clearly demonstrate that MHR-B induces cell adhesion, proliferation and survival, in contrast to the other MHRs. Moreover, MHR-α causes cells to aggregate, decrease proliferation and enter into apoptosis. Cells cultured in standard conditions with 1% soluble MHR-B or MHR-α show the opposite behaviour to the one observed on MHR-B and -α-grafted PS. Fibronectin was similarly adsorbed onto MHR-B and tissue culture polystyrene (TCPS) control, but poorly on MHR-α. The Fn cell binding site (RGD sequence) was more accessible on MHR-B than on TCPS control, but poorly on MHR-α. The disintegrin echistatin inhibited fibroblast adhesion and spreading on MHR-B-grafted PS, which suggests that MHRs control fibroblast behaviour via serum-adhesive proteins. This study provides a basis for the design of intelligently-tailored biomaterial coatings able to induce specific cell functions. © 2008 Elsevier B.V. All rights reserved.
AB - Improved biocompatibility and performance of biomedical devices can be achieved through the incorporation of bioactive molecules on device surfaces. Five structurally distinct pectic polysaccharides (modified hairy regions (MHRs)) were obtained by enzymatic liquefaction of apple (MHR-B, MHR-A and MHR-α), carrot (MHR-C) and potato (MHR-P) cells. Polystyrene (PS) Petri dishes, aminated by a plasma deposition process, were surface modified by the covalent linking of the MHRs. Results clearly demonstrate that MHR-B induces cell adhesion, proliferation and survival, in contrast to the other MHRs. Moreover, MHR-α causes cells to aggregate, decrease proliferation and enter into apoptosis. Cells cultured in standard conditions with 1% soluble MHR-B or MHR-α show the opposite behaviour to the one observed on MHR-B and -α-grafted PS. Fibronectin was similarly adsorbed onto MHR-B and tissue culture polystyrene (TCPS) control, but poorly on MHR-α. The Fn cell binding site (RGD sequence) was more accessible on MHR-B than on TCPS control, but poorly on MHR-α. The disintegrin echistatin inhibited fibroblast adhesion and spreading on MHR-B-grafted PS, which suggests that MHRs control fibroblast behaviour via serum-adhesive proteins. This study provides a basis for the design of intelligently-tailored biomaterial coatings able to induce specific cell functions. © 2008 Elsevier B.V. All rights reserved.
KW - Pectin grafted-biomaterial
KW - Pectin modified hairy region bioactivity
KW - Pectin/fibronectin interaction
U2 - 10.1016/j.bbagen.2008.04.002
DO - 10.1016/j.bbagen.2008.04.002
M3 - Article
SN - 1872-8006
VL - 1780
SP - 995
EP - 1003
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
IS - 7-8
ER -