Enzyme-linked immunosorbent assay of antigens from Candida albicans circulating in infected mice and rabbits: The role of mannan

Richard C. Warren; Malcolm D. Richardson; Les O. White

doi:10.1007/BF00683968

Enzyme-linked immunosorbent assay of antigens from Candida albicans circulating in infected mice and rabbits: The role of mannan

Richard C. Warren, Malcolm D. Richardson, Les O. White

Research output: Contribution to journal › Article › peer-review

Abstract

Various antisera raised either to antigens of Candida albicans or to sub-lethal infections of blastospores (convalescent sera) were tested for their efficacy in diagnosing systemic disease in artifically infected animals. Globulin from convalescent serum, when conjugated with alkaline phosphatase and used in enzyme-linked immunosorbent assays (ELISA), was the only antiserum type which detected circulating Candida-related antigen in the serum of infected animals. Conjugates made from anti-mannan, anti-blastospore or antimycelial globulin did not detect antigen. Mannan did not appear to be related to an antigen produced in sera of experimentally infected mice. The significance of these results in the diagnosis of systemic candidosis is discussed. © 1979 Kluwer Academic Publishers.

Original language	English
Pages (from-to)	179-182
Number of pages	3
Journal	Mycopathologia
Volume	66
Issue number	3
DOIs	https://doi.org/10.1007/BF00683968
Publication status	Published - Jan 1979

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title = "Enzyme-linked immunosorbent assay of antigens from Candida albicans circulating in infected mice and rabbits: The role of mannan",

abstract = "Various antisera raised either to antigens of Candida albicans or to sub-lethal infections of blastospores (convalescent sera) were tested for their efficacy in diagnosing systemic disease in artifically infected animals. Globulin from convalescent serum, when conjugated with alkaline phosphatase and used in enzyme-linked immunosorbent assays (ELISA), was the only antiserum type which detected circulating Candida-related antigen in the serum of infected animals. Conjugates made from anti-mannan, anti-blastospore or antimycelial globulin did not detect antigen. Mannan did not appear to be related to an antigen produced in sera of experimentally infected mice. The significance of these results in the diagnosis of systemic candidosis is discussed. {\textcopyright} 1979 Kluwer Academic Publishers.",

author = "Warren, {Richard C.} and Richardson, {Malcolm D.} and White, {Les O.}",

year = "1979",

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journal = "Mycopathologia",

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AU - Warren, Richard C.

AU - Richardson, Malcolm D.

AU - White, Les O.

PY - 1979/1

Y1 - 1979/1

N2 - Various antisera raised either to antigens of Candida albicans or to sub-lethal infections of blastospores (convalescent sera) were tested for their efficacy in diagnosing systemic disease in artifically infected animals. Globulin from convalescent serum, when conjugated with alkaline phosphatase and used in enzyme-linked immunosorbent assays (ELISA), was the only antiserum type which detected circulating Candida-related antigen in the serum of infected animals. Conjugates made from anti-mannan, anti-blastospore or antimycelial globulin did not detect antigen. Mannan did not appear to be related to an antigen produced in sera of experimentally infected mice. The significance of these results in the diagnosis of systemic candidosis is discussed. © 1979 Kluwer Academic Publishers.

AB - Various antisera raised either to antigens of Candida albicans or to sub-lethal infections of blastospores (convalescent sera) were tested for their efficacy in diagnosing systemic disease in artifically infected animals. Globulin from convalescent serum, when conjugated with alkaline phosphatase and used in enzyme-linked immunosorbent assays (ELISA), was the only antiserum type which detected circulating Candida-related antigen in the serum of infected animals. Conjugates made from anti-mannan, anti-blastospore or antimycelial globulin did not detect antigen. Mannan did not appear to be related to an antigen produced in sera of experimentally infected mice. The significance of these results in the diagnosis of systemic candidosis is discussed. © 1979 Kluwer Academic Publishers.

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DO - 10.1007/BF00683968

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SP - 179

EP - 182

JO - Mycopathologia

JF - Mycopathologia

IS - 3

ER -

Enzyme-linked immunosorbent assay of antigens from Candida albicans circulating in infected mice and rabbits: The role of mannan

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