Establishment of porcine and human expanded potential stem cells

Xuefei Gao; Monika Nowak-Imialek; Xi Chen; Dongsheng Chen; Doris Herrmann; Degong Ruan; Andy Chun Hang Chen; Melanie A Eckersley-Maslin; Shakil Ahmad; Yin Lau Lee; Toshihiro Kobayashi; David Ryan; Jixing Zhong; Jiacheng Zhu; Jian Wu; Guocheng Lan; Stoyan Petkov; Jian Yang; Liliana Antunes; Lia S Campos; Beiyuan Fu; Shengpeng Wang; Yu Yong; Xiaomin Wang; Song-Guo Xue; Liangpeng Ge; Zuohua Liu; Yong Huang; Tao Nie; Peng Li; Donghai Wu; Duanqing Pei; Yi Zhang; Liming Lu; Fengtang Yang; Susan J Kimber; Wolf Reik; Xiangang Zou; Zhouchun Shang; Liangxue Lai; Azim Surani; Patrick P L Tam; Asif Ahmed; William Shu Biu Yeung; Sarah A Teichmann; Heiner Niemann; Pentao Liu

doi:10.1038/s41556-019-0333-2

Establishment of porcine and human expanded potential stem cells

Xuefei Gao, Monika Nowak-Imialek, Xi Chen, Dongsheng Chen, Doris Herrmann, Degong Ruan, Andy Chun Hang Chen, Melanie A Eckersley-Maslin, Shakil Ahmad, Yin Lau Lee, Toshihiro Kobayashi, David Ryan, Jixing Zhong, Jiacheng Zhu, Jian Wu, Guocheng Lan, Stoyan Petkov, Jian Yang, Liliana Antunes, Lia S CamposBeiyuan Fu, Shengpeng Wang, Yu Yong, Xiaomin Wang, Song-Guo Xue, Liangpeng Ge, Zuohua Liu, Yong Huang, Tao Nie, Peng Li, Donghai Wu, Duanqing Pei, Yi Zhang, Liming Lu, Fengtang Yang, Susan J Kimber, Wolf Reik, Xiangang Zou, Zhouchun Shang, Liangxue Lai, Azim Surani, Patrick P L Tam, Asif Ahmed, William Shu Biu Yeung, Sarah A Teichmann, Heiner Niemann, Pentao Liu

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Abstract

We recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the critical molecular pathways that predispose their differentiation. EPSCs had enriched molecular signatures of blastomeres and possessed developmental potency for all embryonic and extra-embryonic cell lineages. Here, we report the derivation of porcine EPSCs, which express key pluripotency genes, are genetically stable, permit genome editing, differentiate to derivatives of the three germ layers in chimeras and produce primordial germ cell-like cells in vitro. Under similar conditions, human embryonic stem cells and induced pluripotent stem cells can be converted, or somatic cells directly reprogrammed, to EPSCs that display the molecular and functional attributes reminiscent of porcine EPSCs. Importantly, trophoblast stem-cell-like cells can be generated from both human and porcine EPSCs. Our pathway-inhibition paradigm thus opens an avenue for generating mammalian pluripotent stem cells, and EPSCs present a unique cellular platform for translational research in biotechnology and regenerative medicine.

Original language	English
Pages (from-to)	687-699
Number of pages	13
Journal	Nature Cell Biology
Volume	21
Issue number	6
Early online date	3 Jun 2019
DOIs	https://doi.org/10.1038/s41556-019-0333-2
Publication status	Published - Jun 2019

Keywords

Animals
Blastomeres/cytology
Cell Differentiation/genetics
Cell Lineage/genetics
Cellular Reprogramming/genetics
Embryonic Stem Cells/cytology
Germ Layers/growth & development
Humans
Induced Pluripotent Stem Cells/cytology
Mice
Pluripotent Stem Cells/cytology
Regenerative Medicine
Signal Transduction/genetics
Swine
Trophoblasts/cytology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/s41556-019-0333-2

Merged%20PDF%20porcine%20and%20human%20EPSCAccepted author manuscript, 5.25 MB

Cite this

Gao, X., Nowak-Imialek, M., Chen, X., Chen, D., Herrmann, D., Ruan, D., Chen, A. C. H., Eckersley-Maslin, M. A., Ahmad, S., Lee, Y. L., Kobayashi, T., Ryan, D., Zhong, J., Zhu, J., Wu, J., Lan, G., Petkov, S., Yang, J., Antunes, L., ... Liu, P. (2019). Establishment of porcine and human expanded potential stem cells. Nature Cell Biology, 21(6), 687-699. https://doi.org/10.1038/s41556-019-0333-2

@article{7107b965b0a6465ca3d85b93339ef3e4,

title = "Establishment of porcine and human expanded potential stem cells",

abstract = "We recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the critical molecular pathways that predispose their differentiation. EPSCs had enriched molecular signatures of blastomeres and possessed developmental potency for all embryonic and extra-embryonic cell lineages. Here, we report the derivation of porcine EPSCs, which express key pluripotency genes, are genetically stable, permit genome editing, differentiate to derivatives of the three germ layers in chimeras and produce primordial germ cell-like cells in vitro. Under similar conditions, human embryonic stem cells and induced pluripotent stem cells can be converted, or somatic cells directly reprogrammed, to EPSCs that display the molecular and functional attributes reminiscent of porcine EPSCs. Importantly, trophoblast stem-cell-like cells can be generated from both human and porcine EPSCs. Our pathway-inhibition paradigm thus opens an avenue for generating mammalian pluripotent stem cells, and EPSCs present a unique cellular platform for translational research in biotechnology and regenerative medicine.",

keywords = "Animals, Blastomeres/cytology, Cell Differentiation/genetics, Cell Lineage/genetics, Cellular Reprogramming/genetics, Embryonic Stem Cells/cytology, Germ Layers/growth & development, Humans, Induced Pluripotent Stem Cells/cytology, Mice, Pluripotent Stem Cells/cytology, Regenerative Medicine, Signal Transduction/genetics, Swine, Trophoblasts/cytology",

author = "Xuefei Gao and Monika Nowak-Imialek and Xi Chen and Dongsheng Chen and Doris Herrmann and Degong Ruan and Chen, {Andy Chun Hang} and Eckersley-Maslin, {Melanie A} and Shakil Ahmad and Lee, {Yin Lau} and Toshihiro Kobayashi and David Ryan and Jixing Zhong and Jiacheng Zhu and Jian Wu and Guocheng Lan and Stoyan Petkov and Jian Yang and Liliana Antunes and Campos, {Lia S} and Beiyuan Fu and Shengpeng Wang and Yu Yong and Xiaomin Wang and Song-Guo Xue and Liangpeng Ge and Zuohua Liu and Yong Huang and Tao Nie and Peng Li and Donghai Wu and Duanqing Pei and Yi Zhang and Liming Lu and Fengtang Yang and Kimber, {Susan J} and Wolf Reik and Xiangang Zou and Zhouchun Shang and Liangxue Lai and Azim Surani and Tam, {Patrick P L} and Asif Ahmed and Yeung, {William Shu Biu} and Teichmann, {Sarah A} and Heiner Niemann and Pentao Liu",

year = "2019",

month = jun,

doi = "10.1038/s41556-019-0333-2",

language = "English",

volume = "21",

pages = "687--699",

journal = "Nature Cell Biology",

issn = "1465-7392",

publisher = "Springer Nature",

number = "6",

}

Gao, X, Nowak-Imialek, M, Chen, X, Chen, D, Herrmann, D, Ruan, D, Chen, ACH, Eckersley-Maslin, MA, Ahmad, S, Lee, YL, Kobayashi, T, Ryan, D, Zhong, J, Zhu, J, Wu, J, Lan, G, Petkov, S, Yang, J, Antunes, L, Campos, LS, Fu, B, Wang, S, Yong, Y, Wang, X, Xue, S-G, Ge, L, Liu, Z, Huang, Y, Nie, T, Li, P, Wu, D, Pei, D, Zhang, Y, Lu, L, Yang, F, Kimber, SJ, Reik, W, Zou, X, Shang, Z, Lai, L, Surani, A, Tam, PPL, Ahmed, A, Yeung, WSB, Teichmann, SA, Niemann, H & Liu, P 2019, 'Establishment of porcine and human expanded potential stem cells', Nature Cell Biology, vol. 21, no. 6, pp. 687-699. https://doi.org/10.1038/s41556-019-0333-2

TY - JOUR

T1 - Establishment of porcine and human expanded potential stem cells

AU - Gao, Xuefei

AU - Nowak-Imialek, Monika

AU - Chen, Xi

AU - Chen, Dongsheng

AU - Herrmann, Doris

AU - Ruan, Degong

AU - Chen, Andy Chun Hang

AU - Eckersley-Maslin, Melanie A

AU - Ahmad, Shakil

AU - Lee, Yin Lau

AU - Kobayashi, Toshihiro

AU - Ryan, David

AU - Zhong, Jixing

AU - Zhu, Jiacheng

AU - Wu, Jian

AU - Lan, Guocheng

AU - Petkov, Stoyan

AU - Yang, Jian

AU - Antunes, Liliana

AU - Campos, Lia S

AU - Fu, Beiyuan

AU - Wang, Shengpeng

AU - Yong, Yu

AU - Wang, Xiaomin

AU - Xue, Song-Guo

AU - Ge, Liangpeng

AU - Liu, Zuohua

AU - Huang, Yong

AU - Nie, Tao

AU - Li, Peng

AU - Wu, Donghai

AU - Pei, Duanqing

AU - Zhang, Yi

AU - Lu, Liming

AU - Yang, Fengtang

AU - Kimber, Susan J

AU - Reik, Wolf

AU - Zou, Xiangang

AU - Shang, Zhouchun

AU - Lai, Liangxue

AU - Surani, Azim

AU - Tam, Patrick P L

AU - Ahmed, Asif

AU - Yeung, William Shu Biu

AU - Teichmann, Sarah A

AU - Niemann, Heiner

AU - Liu, Pentao

PY - 2019/6

Y1 - 2019/6

N2 - We recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the critical molecular pathways that predispose their differentiation. EPSCs had enriched molecular signatures of blastomeres and possessed developmental potency for all embryonic and extra-embryonic cell lineages. Here, we report the derivation of porcine EPSCs, which express key pluripotency genes, are genetically stable, permit genome editing, differentiate to derivatives of the three germ layers in chimeras and produce primordial germ cell-like cells in vitro. Under similar conditions, human embryonic stem cells and induced pluripotent stem cells can be converted, or somatic cells directly reprogrammed, to EPSCs that display the molecular and functional attributes reminiscent of porcine EPSCs. Importantly, trophoblast stem-cell-like cells can be generated from both human and porcine EPSCs. Our pathway-inhibition paradigm thus opens an avenue for generating mammalian pluripotent stem cells, and EPSCs present a unique cellular platform for translational research in biotechnology and regenerative medicine.

AB - We recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the critical molecular pathways that predispose their differentiation. EPSCs had enriched molecular signatures of blastomeres and possessed developmental potency for all embryonic and extra-embryonic cell lineages. Here, we report the derivation of porcine EPSCs, which express key pluripotency genes, are genetically stable, permit genome editing, differentiate to derivatives of the three germ layers in chimeras and produce primordial germ cell-like cells in vitro. Under similar conditions, human embryonic stem cells and induced pluripotent stem cells can be converted, or somatic cells directly reprogrammed, to EPSCs that display the molecular and functional attributes reminiscent of porcine EPSCs. Importantly, trophoblast stem-cell-like cells can be generated from both human and porcine EPSCs. Our pathway-inhibition paradigm thus opens an avenue for generating mammalian pluripotent stem cells, and EPSCs present a unique cellular platform for translational research in biotechnology and regenerative medicine.

KW - Animals

KW - Blastomeres/cytology

KW - Cell Differentiation/genetics

KW - Cell Lineage/genetics

KW - Cellular Reprogramming/genetics

KW - Embryonic Stem Cells/cytology

KW - Germ Layers/growth & development

KW - Humans

KW - Induced Pluripotent Stem Cells/cytology

KW - Mice

KW - Pluripotent Stem Cells/cytology

KW - Regenerative Medicine

KW - Signal Transduction/genetics

KW - Swine

KW - Trophoblasts/cytology

U2 - 10.1038/s41556-019-0333-2

DO - 10.1038/s41556-019-0333-2

M3 - Article

C2 - 31160711

VL - 21

SP - 687

EP - 699

JO - Nature Cell Biology

JF - Nature Cell Biology

SN - 1465-7392

IS - 6

ER -

Establishment of porcine and human expanded potential stem cells

Abstract

Keywords

UN SDGs

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