Estrogen receptor alpha interacts with 17β-hydroxysteroid dehydrogenase type 10 in mitochondria

Virginija Jazbutyte; Franz Kehl; Ludwig Neyses; Theo Pelzer

doi:10.1016/j.bbrc.2009.04.139

Estrogen receptor alpha interacts with 17β-hydroxysteroid dehydrogenase type 10 in mitochondria

Virginija Jazbutyte, Franz Kehl, Ludwig Neyses, Theo Pelzer

Research output: Contribution to journal › Article › peer-review

Abstract

Estrogen receptor alpha (ERα) is present in the nucleus, the cytosol and in mitochondria. The rat ERα ligand binding domain was employed as bait in a bacterial two-hybrid screening of a human heart cDNA library to detect novel protein-protein interaction partners of ERα in the heart. 17β-Hydroxysteroid dehydrogenase type 10 (17β-HSD10), which converts potent (17β-estradiol) to less potent estrogens (estrone), co-localized with 17β-HSD10 in the mitochondria of rat cardiac myocytes. GST pull-down experiments confirmed the interaction of ERα and 17β-HSD10. These findings suggest that the ERα estrogen receptor might be involved in regulating intracellular estrogen levels by modulating 17β-HSD10 activity. © 2009 Elsevier Inc.

Original language	English
Pages (from-to)	450-454
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	384
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2009.04.139
Publication status	Published - 10 Jul 2009

Keywords

17β-Hydroxysteroid dehydrogenase type 10
Estrogen receptor alpha
Heart
Mitochondria

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10.1016/j.bbrc.2009.04.139

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title = "Estrogen receptor alpha interacts with 17β-hydroxysteroid dehydrogenase type 10 in mitochondria",

abstract = "Estrogen receptor alpha (ERα) is present in the nucleus, the cytosol and in mitochondria. The rat ERα ligand binding domain was employed as bait in a bacterial two-hybrid screening of a human heart cDNA library to detect novel protein-protein interaction partners of ERα in the heart. 17β-Hydroxysteroid dehydrogenase type 10 (17β-HSD10), which converts potent (17β-estradiol) to less potent estrogens (estrone), co-localized with 17β-HSD10 in the mitochondria of rat cardiac myocytes. GST pull-down experiments confirmed the interaction of ERα and 17β-HSD10. These findings suggest that the ERα estrogen receptor might be involved in regulating intracellular estrogen levels by modulating 17β-HSD10 activity. {\textcopyright} 2009 Elsevier Inc.",

keywords = "17β-Hydroxysteroid dehydrogenase type 10, Estrogen receptor alpha, Heart, Mitochondria",

author = "Virginija Jazbutyte and Franz Kehl and Ludwig Neyses and Theo Pelzer",

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doi = "10.1016/j.bbrc.2009.04.139",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Estrogen receptor alpha interacts with 17β-hydroxysteroid dehydrogenase type 10 in mitochondria

AU - Jazbutyte, Virginija

AU - Kehl, Franz

AU - Neyses, Ludwig

AU - Pelzer, Theo

PY - 2009/7/10

Y1 - 2009/7/10

N2 - Estrogen receptor alpha (ERα) is present in the nucleus, the cytosol and in mitochondria. The rat ERα ligand binding domain was employed as bait in a bacterial two-hybrid screening of a human heart cDNA library to detect novel protein-protein interaction partners of ERα in the heart. 17β-Hydroxysteroid dehydrogenase type 10 (17β-HSD10), which converts potent (17β-estradiol) to less potent estrogens (estrone), co-localized with 17β-HSD10 in the mitochondria of rat cardiac myocytes. GST pull-down experiments confirmed the interaction of ERα and 17β-HSD10. These findings suggest that the ERα estrogen receptor might be involved in regulating intracellular estrogen levels by modulating 17β-HSD10 activity. © 2009 Elsevier Inc.

AB - Estrogen receptor alpha (ERα) is present in the nucleus, the cytosol and in mitochondria. The rat ERα ligand binding domain was employed as bait in a bacterial two-hybrid screening of a human heart cDNA library to detect novel protein-protein interaction partners of ERα in the heart. 17β-Hydroxysteroid dehydrogenase type 10 (17β-HSD10), which converts potent (17β-estradiol) to less potent estrogens (estrone), co-localized with 17β-HSD10 in the mitochondria of rat cardiac myocytes. GST pull-down experiments confirmed the interaction of ERα and 17β-HSD10. These findings suggest that the ERα estrogen receptor might be involved in regulating intracellular estrogen levels by modulating 17β-HSD10 activity. © 2009 Elsevier Inc.

KW - 17β-Hydroxysteroid dehydrogenase type 10

KW - Estrogen receptor alpha

KW - Heart

KW - Mitochondria

U2 - 10.1016/j.bbrc.2009.04.139

DO - 10.1016/j.bbrc.2009.04.139

M3 - Article

C2 - 19422801

SN - 1090-2104

VL - 384

SP - 450

EP - 454

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Estrogen receptor alpha interacts with 17β-hydroxysteroid dehydrogenase type 10 in mitochondria

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