Abstract
Introduction
To further evaluate the long-term antibiofilm and fluorescence properties as well as enamel demineralization prevention ability, removal efficiency, and biocompatibility of an orthodontic adhesive modified with ZnO quantum dots (ZnQDs).
Methods
ZnQDs were synthesized and characterized by TEM and fluorescence observation.
MIC and MBC of ZnQDs against S. mutans were evaluated. ZnQDs were incorporated into Transbond XT adhesive paste with 20 wt% to form the multifunctional orthodontic adhesive QDA20. Long-term antibiofilm capability and fluorescence property were evaluated after saliva storage aging. A biofilm demineralization model was constructed and the enamel demineralization degree was evaluated by color analysis, Raman analysis and micro-CT. Bracket bonding and debonding procedures were performed on a head simulator and the effectiveness of adhesive removal was assessed. Subcutaneous tissue, blood and organ compatibility assay was performed on a rat subcutaneous tissue implant model.
Results
ZnQDs had a diameter of around 5 nm, and the MIC and MBC against S. mutans were 0.3215 mg/mL and 1.25 mg/mL. ZnQDs showed long-lasting antibiofilm and
fluorescent properties and could reduce the color change and mineral loss of enamel during the biofilm demineralization process. On the head simulator, QDA20 could help the operator remove adhesive more thoroughly without damaging enamel. Histological analysis of subcutaneous tissue and organs, and blood analysis proved that QDA20 was well-biocompatible.
Conclusions
ZnQDs showed excellent antibiofilm and fluorescent properties and thus could be a multifunctional adhesive to overcome the two major challenges of enamel
demineralization and difficulty in recognizing adhesives during fixed orthodontic
treatment.
To further evaluate the long-term antibiofilm and fluorescence properties as well as enamel demineralization prevention ability, removal efficiency, and biocompatibility of an orthodontic adhesive modified with ZnO quantum dots (ZnQDs).
Methods
ZnQDs were synthesized and characterized by TEM and fluorescence observation.
MIC and MBC of ZnQDs against S. mutans were evaluated. ZnQDs were incorporated into Transbond XT adhesive paste with 20 wt% to form the multifunctional orthodontic adhesive QDA20. Long-term antibiofilm capability and fluorescence property were evaluated after saliva storage aging. A biofilm demineralization model was constructed and the enamel demineralization degree was evaluated by color analysis, Raman analysis and micro-CT. Bracket bonding and debonding procedures were performed on a head simulator and the effectiveness of adhesive removal was assessed. Subcutaneous tissue, blood and organ compatibility assay was performed on a rat subcutaneous tissue implant model.
Results
ZnQDs had a diameter of around 5 nm, and the MIC and MBC against S. mutans were 0.3215 mg/mL and 1.25 mg/mL. ZnQDs showed long-lasting antibiofilm and
fluorescent properties and could reduce the color change and mineral loss of enamel during the biofilm demineralization process. On the head simulator, QDA20 could help the operator remove adhesive more thoroughly without damaging enamel. Histological analysis of subcutaneous tissue and organs, and blood analysis proved that QDA20 was well-biocompatible.
Conclusions
ZnQDs showed excellent antibiofilm and fluorescent properties and thus could be a multifunctional adhesive to overcome the two major challenges of enamel
demineralization and difficulty in recognizing adhesives during fixed orthodontic
treatment.
| Original language | English |
|---|---|
| Journal | American Journal of Orthodontics and Dentofacial Orthopedics |
| Early online date | 22 Mar 2025 |
| DOIs | |
| Publication status | E-pub ahead of print - 22 Mar 2025 |
Keywords
- Orthodontic
- Adhesives
- Antibacterial
- Fluorescence
- Enamel demineralization