Everyone loves an underdog: metabolic engineering of the xylose oxidative pathway in recombinant microorganisms

Kris Niño G. Valdehuesa; Kristine Rose M. Ramos; Grace M. Nisola; Angelo B. Bañares; Rhudith B. Cabulong; Won Keun Lee; Huaiwei Liu; Wook Jin Chung

doi:10.1007/s00253-018-9186-z

Everyone loves an underdog: metabolic engineering of the xylose oxidative pathway in recombinant microorganisms

Kris Niño G. Valdehuesa, Kristine Rose M. Ramos, Grace M. Nisola, Angelo B. Bañares, Rhudith B. Cabulong, Won Keun Lee, Huaiwei Liu, Wook Jin Chung^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

Abstract

The d-xylose oxidative pathway (XOP) has recently been employed in several recombinant microorganisms for growth or for the production of several valuable compounds. The XOP is initiated by d-xylose oxidation to d-xylonolactone, which is then hydrolyzed into d-xylonic acid. d-Xylonic acid is then dehydrated to form 2-keto-3-deoxy-d-xylonic acid, which may be further dehydrated then oxidized into α-ketoglutarate or undergo aldol cleavage to form pyruvate and glycolaldehyde. This review introduces a brief discussion about XOP and its discovery in bacteria and archaea, such as Caulobacter crescentus and Haloferax volcanii. Furthermore, the current advances in the metabolic engineering of recombinant strains employing the XOP are discussed. This includes utilization of XOP for the production of diols, triols, and short-chain organic acids in Escherichia coli, Saccharomyces cerevisiae, and Corynebacterium glutamicum. Improving the d-xylose uptake, growth yields, and product titer through several metabolic engineering techniques bring some of these recombinant strains close to industrial viability. However, more developments are still needed to optimize the XOP pathway in the host strains, particularly in the minimization of by-product formation.

Original language	English
Pages (from-to)	7703-7716
Number of pages	14
Journal	Applied microbiology and biotechnology
Volume	102
Issue number	18
Early online date	12 Jul 2018
DOIs	https://doi.org/10.1007/s00253-018-9186-z
Publication status	Published - 1 Sept 2018
Externally published	Yes

Keywords

d-xylose
Dahms pathway
Metabolic engineering
Nonphosphorylative metabolism
Weimberg pathway
Xylose oxidative pathway

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10.1007/s00253-018-9186-z

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title = "Everyone loves an underdog: metabolic engineering of the xylose oxidative pathway in recombinant microorganisms",

abstract = "The d-xylose oxidative pathway (XOP) has recently been employed in several recombinant microorganisms for growth or for the production of several valuable compounds. The XOP is initiated by d-xylose oxidation to d-xylonolactone, which is then hydrolyzed into d-xylonic acid. d-Xylonic acid is then dehydrated to form 2-keto-3-deoxy-d-xylonic acid, which may be further dehydrated then oxidized into α-ketoglutarate or undergo aldol cleavage to form pyruvate and glycolaldehyde. This review introduces a brief discussion about XOP and its discovery in bacteria and archaea, such as Caulobacter crescentus and Haloferax volcanii. Furthermore, the current advances in the metabolic engineering of recombinant strains employing the XOP are discussed. This includes utilization of XOP for the production of diols, triols, and short-chain organic acids in Escherichia coli, Saccharomyces cerevisiae, and Corynebacterium glutamicum. Improving the d-xylose uptake, growth yields, and product titer through several metabolic engineering techniques bring some of these recombinant strains close to industrial viability. However, more developments are still needed to optimize the XOP pathway in the host strains, particularly in the minimization of by-product formation.",

keywords = "d-xylose, Dahms pathway, Metabolic engineering, Nonphosphorylative metabolism, Weimberg pathway, Xylose oxidative pathway",

author = "Valdehuesa, {Kris Ni{\~n}o G.} and Ramos, {Kristine Rose M.} and Nisola, {Grace M.} and Ba{\~n}ares, {Angelo B.} and Cabulong, {Rhudith B.} and Lee, {Won Keun} and Huaiwei Liu and Chung, {Wook Jin}",

year = "2018",

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doi = "10.1007/s00253-018-9186-z",

language = "English",

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journal = "Applied microbiology and biotechnology",

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TY - JOUR

T1 - Everyone loves an underdog

T2 - metabolic engineering of the xylose oxidative pathway in recombinant microorganisms

AU - Valdehuesa, Kris Niño G.

AU - Ramos, Kristine Rose M.

AU - Nisola, Grace M.

AU - Bañares, Angelo B.

AU - Cabulong, Rhudith B.

AU - Lee, Won Keun

AU - Liu, Huaiwei

AU - Chung, Wook Jin

PY - 2018/9/1

Y1 - 2018/9/1

N2 - The d-xylose oxidative pathway (XOP) has recently been employed in several recombinant microorganisms for growth or for the production of several valuable compounds. The XOP is initiated by d-xylose oxidation to d-xylonolactone, which is then hydrolyzed into d-xylonic acid. d-Xylonic acid is then dehydrated to form 2-keto-3-deoxy-d-xylonic acid, which may be further dehydrated then oxidized into α-ketoglutarate or undergo aldol cleavage to form pyruvate and glycolaldehyde. This review introduces a brief discussion about XOP and its discovery in bacteria and archaea, such as Caulobacter crescentus and Haloferax volcanii. Furthermore, the current advances in the metabolic engineering of recombinant strains employing the XOP are discussed. This includes utilization of XOP for the production of diols, triols, and short-chain organic acids in Escherichia coli, Saccharomyces cerevisiae, and Corynebacterium glutamicum. Improving the d-xylose uptake, growth yields, and product titer through several metabolic engineering techniques bring some of these recombinant strains close to industrial viability. However, more developments are still needed to optimize the XOP pathway in the host strains, particularly in the minimization of by-product formation.

AB - The d-xylose oxidative pathway (XOP) has recently been employed in several recombinant microorganisms for growth or for the production of several valuable compounds. The XOP is initiated by d-xylose oxidation to d-xylonolactone, which is then hydrolyzed into d-xylonic acid. d-Xylonic acid is then dehydrated to form 2-keto-3-deoxy-d-xylonic acid, which may be further dehydrated then oxidized into α-ketoglutarate or undergo aldol cleavage to form pyruvate and glycolaldehyde. This review introduces a brief discussion about XOP and its discovery in bacteria and archaea, such as Caulobacter crescentus and Haloferax volcanii. Furthermore, the current advances in the metabolic engineering of recombinant strains employing the XOP are discussed. This includes utilization of XOP for the production of diols, triols, and short-chain organic acids in Escherichia coli, Saccharomyces cerevisiae, and Corynebacterium glutamicum. Improving the d-xylose uptake, growth yields, and product titer through several metabolic engineering techniques bring some of these recombinant strains close to industrial viability. However, more developments are still needed to optimize the XOP pathway in the host strains, particularly in the minimization of by-product formation.

KW - d-xylose

KW - Dahms pathway

KW - Metabolic engineering

KW - Nonphosphorylative metabolism

KW - Weimberg pathway

KW - Xylose oxidative pathway

U2 - 10.1007/s00253-018-9186-z

DO - 10.1007/s00253-018-9186-z

M3 - Review article

C2 - 30003296

AN - SCOPUS:85049804403

SN - 0175-7598

VL - 102

SP - 7703

EP - 7716

JO - Applied microbiology and biotechnology

JF - Applied microbiology and biotechnology

IS - 18

ER -

Everyone loves an underdog: metabolic engineering of the xylose oxidative pathway in recombinant microorganisms

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Keywords

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