Evidence for metabolism of o-xylene by simultaneous ring and methyl group oxidation in a new soil isolate

S. R. Bickerdike, R. A. Holt, G. M. Stephens

    Research output: Contribution to journalArticlepeer-review

    Abstract

    An o-xylene-utilizing Rhodococcus, strain B3, was isolated from enrichments with o-xylene. The pathway for o-xylene degradation was investigated by simultaneous adaptation experiments, studies of product formation by a mutant and fortuitous oxidation studies using trimethylbenzene isomers as substrates. Two pathways were found to operate simultaneously and both were inducible. The first pathway involved the oxidation of a methyl group to form 2-methylbenzyl alcohol, followed by oxidation via the corresponding acid to 3-methylcatechol. The second pathway involved oxidation of the aromatic ring to form a dimethylcatechol. The bulk of the evidence suggests that the initial reaction was catalysed by a monooxygenase rather than a dioxygenase, and that 2,3-dimethylphenol was produced as an intermediate.
    Original languageEnglish
    Pages (from-to)2321-2329
    Number of pages8
    JournalMicrobiology
    Volume143
    Issue number7
    Publication statusPublished - Jul 1997

    Keywords

    • cis-glycols
    • Dimethylcatechols
    • Dimethylphenols
    • o-xylene metabolism

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