Exploring subcellular imaging on the buncher-ToF J105 3D chemical imager

S. Rabbani; J. S. Fletcher; N. P. Lockyer; J. C. Vickerman

doi:10.1002/sia.3457

Exploring subcellular imaging on the buncher-ToF J105 3D chemical imager

S. Rabbani, J. S. Fletcher, N. P. Lockyer, J. C. Vickerman

Research output: Contribution to journal › Article › peer-review

Abstract

Imaging Mass Spectrometry is emerging as a promising technique to further the understanding of disease and treatment. ToF-SIMS is also uniquely placed to become a powerful tool in this field due to its chemical specificity and the ability to perform high spatial resolution imaging. In this paper advances in single cell imaging at a sub-cellular level in 2D and 3D are explored using a novel SIMS instrument based on a buncher-ToF configuration, the J105 3D Chemical Imager. The data shows molecular localisation on a sub-cellular scale, over a suitable timeframe, whilst sustaining high mass resolution. The cells are imaged using a 40 keV C60+ dc beam and a clear differentiation between the material within the nuclei and lipid membrane can be made. In addition challenges in data manipulation and interpretation are discussed. Investigation of cell preparation suggests that the frozen hydrated approach may be beneficial. Copyright © 2010 John Wiley & Sons, Ltd.

Original language	English
Pages (from-to)	380-384
Number of pages	4
Journal	Surface and Interface Analysis
Volume	43
Issue number	1-2
DOIs	https://doi.org/10.1002/sia.3457
Publication status	Published - Jan 2011

Keywords

2D and 3D imaging
HeLa M single cells
high-mass resolution
J105 3D chemical imager
sub-cellular imaging

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10.1002/sia.3457

http://doi.wiley.com/10.1002/sia.3457

Cite this

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title = "Exploring subcellular imaging on the buncher-ToF J105 3D chemical imager",

abstract = "Imaging Mass Spectrometry is emerging as a promising technique to further the understanding of disease and treatment. ToF-SIMS is also uniquely placed to become a powerful tool in this field due to its chemical specificity and the ability to perform high spatial resolution imaging. In this paper advances in single cell imaging at a sub-cellular level in 2D and 3D are explored using a novel SIMS instrument based on a buncher-ToF configuration, the J105 3D Chemical Imager. The data shows molecular localisation on a sub-cellular scale, over a suitable timeframe, whilst sustaining high mass resolution. The cells are imaged using a 40 keV C60+ dc beam and a clear differentiation between the material within the nuclei and lipid membrane can be made. In addition challenges in data manipulation and interpretation are discussed. Investigation of cell preparation suggests that the frozen hydrated approach may be beneficial. Copyright {\textcopyright} 2010 John Wiley & Sons, Ltd.",

keywords = "2D and 3D imaging, HeLa M single cells, high-mass resolution, J105 3D chemical imager, sub-cellular imaging",

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year = "2011",

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language = "English",

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AU - Rabbani, S.

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AU - Vickerman, J. C.

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KW - 2D and 3D imaging

KW - HeLa M single cells

KW - high-mass resolution

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U2 - 10.1002/sia.3457

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Exploring subcellular imaging on the buncher-ToF J105 3D chemical imager

Abstract

Keywords

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