Expression and characterisation of a very-late antigen-4 (alpha 4 beta 1) integrin-binding fragment of vascular cell-adhesion molecule-1

T J Dudgeon, M J Bottomley, P C Driscoll, M J Humphries, A P Mould, G I Wingfield, J M Clements

Research output: Contribution to journalArticlepeer-review

Abstract

We have used an Escherichia coli expression system to produce forms of vascular cell-adhesion molecule-1 (VCAM-1) containing the first two and three supposed immunoglobulin-like domains. A form consisting of the first two domains of VCAM-1 is shown to promote very-late antigen-4-dependent spreading of a melanoma cell line comparable to that found for the equivalent region in the full seven-domain form. Preliminary structural analysis by CD and NMR is consistent with an immunoglobulin fold which is predicted from sequence comparison studies.

Original languageEnglish
Pages (from-to)517-23
Number of pages7
JournalEuropean Journal of Biochemistry / FEBS
Volume226
Issue number2
Publication statusPublished - 1 Dec 1994

Keywords

  • Binding Sites
  • Cell Adhesion
  • Cell Adhesion Molecules
  • Circular Dichroism
  • Escherichia coli
  • Gene Expression
  • Humans
  • Integrin alpha4beta1
  • Integrins
  • Magnetic Resonance Spectroscopy
  • Melanoma
  • Peptide Fragments
  • Protein Structure, Secondary
  • Receptors, Very Late Antigen
  • Recombinant Proteins
  • Structure-Activity Relationship
  • Tumor Cells, Cultured
  • Vascular Cell Adhesion Molecule-1

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