Expression and downregulation of cytotoxic cell protease 1 or Granzyme 'B' transcripts during myeloid differentiation of interleukin-3-dependent murine stem cell lines

I. N. Hampson, M. A. Cross, C. M. Heyworth, L. Fairbairn, E. Spooncer, G. J. Cowling, T. M. Dexter

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Using the technique of differential cDNA library screening, we have molecularly cloned a gene that is highly expressed in an undifferentiated myeloid multipotent and growth factor-dependent stem cell line (FDCP-Mix) and that downregulates as these cells are induced to differentiate along monocytic, granulocytic, and erythroid cell lineages. Sequence analysis of this gene has shown homology with a previously cloned gene, cytotoxic cell protease 1 (CCP1 or Granzyme 'B'), that has been shown to be expressed only in thymocytes, activated T cells, a mast cell line, and peritoneal exudate leukocytes. In situ hybridization, Northern blot analysis, and nuclear run-off assay has confirmed that expression of CCP1 is restricted to the phenotypically primitive multipotent undifferentiated FDCP-Mix cells that are undergoing self-renewal in the presence of growth factors such as interleukin-3. © 1992 by The American Society of Hematology.
    Original languageEnglish
    Pages (from-to)3097-3105
    Number of pages8
    JournalBlood
    Volume80
    Issue number12
    Publication statusPublished - 15 Dec 1992

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