Expression of a sodium bicarbonate cotransporter in human parotid salivary glands

K. Park; P. T. Hurley; E. Roussa; G. J. Cooper; C. P. Smith; F. Thévenod; M. C. Steward; R. M. Case

doi:10.1016/S0003-9969(01)00098-X

Expression of a sodium bicarbonate cotransporter in human parotid salivary glands

K. Park, P. T. Hurley, E. Roussa, G. J. Cooper, C. P. Smith, F. Thévenod, M. C. Steward, R. M. Case

Research output: Contribution to journal › Article › peer-review

Abstract

The human parotid gland secretes much of the bicarbonate that enters the mouth. Prompted by studies of animal models, this study sought evidence for the expression of a functional Na+-HCO3- cotransporter (NBC) in human parotid acinar cells. Microfluorometric measurements of intracellular pH in isolated acini showed that the recovery from an acid load was achieved in part by HCO3- uptake via a Na+-dependent, DIDS-sensitive mechanism. By reverse transcriptase-polymerase chain reaction, a full-length NBC1 clone was obtained showing more than 99% homology with the human pancreatic isoform hpNBC 1. Expressed in Xenopus oocytes, the electrogenicity of the transporter was detected as an inwardly directed, Na+- and HCO3--dependent flux of negative charge. Immunohistochemistry using antibodies raised to NBC1 showed strong staining of the basolateral membrane of the acinar cells. Therefore, it was concluded that a functional electrogenic Na+-HCO3- cotransporter is expressed in the human parotid gland, and that it contributes to pH regulation in the acinar cells and could play a significant part in salivary secretion. © 2002 Elsevier Science Ltd. All rights reserved.

Original language	English
Pages (from-to)	1-9
Number of pages	8
Journal	Archives of oral biology
Volume	47
Issue number	1
DOIs	https://doi.org/10.1016/S0003-9969(01)00098-X
Publication status	Published - 2002

Keywords

Bicarbonate secretion
Intracellular pH
NBC

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10.1016/S0003-9969(01)00098-X

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title = "Expression of a sodium bicarbonate cotransporter in human parotid salivary glands",

abstract = "The human parotid gland secretes much of the bicarbonate that enters the mouth. Prompted by studies of animal models, this study sought evidence for the expression of a functional Na+-HCO3- cotransporter (NBC) in human parotid acinar cells. Microfluorometric measurements of intracellular pH in isolated acini showed that the recovery from an acid load was achieved in part by HCO3- uptake via a Na+-dependent, DIDS-sensitive mechanism. By reverse transcriptase-polymerase chain reaction, a full-length NBC1 clone was obtained showing more than 99% homology with the human pancreatic isoform hpNBC 1. Expressed in Xenopus oocytes, the electrogenicity of the transporter was detected as an inwardly directed, Na+- and HCO3--dependent flux of negative charge. Immunohistochemistry using antibodies raised to NBC1 showed strong staining of the basolateral membrane of the acinar cells. Therefore, it was concluded that a functional electrogenic Na+-HCO3- cotransporter is expressed in the human parotid gland, and that it contributes to pH regulation in the acinar cells and could play a significant part in salivary secretion. {\textcopyright} 2002 Elsevier Science Ltd. All rights reserved.",

keywords = "Bicarbonate secretion, Intracellular pH, NBC",

author = "K. Park and Hurley, {P. T.} and E. Roussa and Cooper, {G. J.} and Smith, {C. P.} and F. Th{\'e}venod and Steward, {M. C.} and Case, {R. M.}",

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AU - Park, K.

AU - Hurley, P. T.

AU - Roussa, E.

AU - Cooper, G. J.

AU - Smith, C. P.

AU - Thévenod, F.

AU - Steward, M. C.

AU - Case, R. M.

PY - 2002

Y1 - 2002

N2 - The human parotid gland secretes much of the bicarbonate that enters the mouth. Prompted by studies of animal models, this study sought evidence for the expression of a functional Na+-HCO3- cotransporter (NBC) in human parotid acinar cells. Microfluorometric measurements of intracellular pH in isolated acini showed that the recovery from an acid load was achieved in part by HCO3- uptake via a Na+-dependent, DIDS-sensitive mechanism. By reverse transcriptase-polymerase chain reaction, a full-length NBC1 clone was obtained showing more than 99% homology with the human pancreatic isoform hpNBC 1. Expressed in Xenopus oocytes, the electrogenicity of the transporter was detected as an inwardly directed, Na+- and HCO3--dependent flux of negative charge. Immunohistochemistry using antibodies raised to NBC1 showed strong staining of the basolateral membrane of the acinar cells. Therefore, it was concluded that a functional electrogenic Na+-HCO3- cotransporter is expressed in the human parotid gland, and that it contributes to pH regulation in the acinar cells and could play a significant part in salivary secretion. © 2002 Elsevier Science Ltd. All rights reserved.

AB - The human parotid gland secretes much of the bicarbonate that enters the mouth. Prompted by studies of animal models, this study sought evidence for the expression of a functional Na+-HCO3- cotransporter (NBC) in human parotid acinar cells. Microfluorometric measurements of intracellular pH in isolated acini showed that the recovery from an acid load was achieved in part by HCO3- uptake via a Na+-dependent, DIDS-sensitive mechanism. By reverse transcriptase-polymerase chain reaction, a full-length NBC1 clone was obtained showing more than 99% homology with the human pancreatic isoform hpNBC 1. Expressed in Xenopus oocytes, the electrogenicity of the transporter was detected as an inwardly directed, Na+- and HCO3--dependent flux of negative charge. Immunohistochemistry using antibodies raised to NBC1 showed strong staining of the basolateral membrane of the acinar cells. Therefore, it was concluded that a functional electrogenic Na+-HCO3- cotransporter is expressed in the human parotid gland, and that it contributes to pH regulation in the acinar cells and could play a significant part in salivary secretion. © 2002 Elsevier Science Ltd. All rights reserved.

KW - Bicarbonate secretion

KW - Intracellular pH

KW - NBC

U2 - 10.1016/S0003-9969(01)00098-X

DO - 10.1016/S0003-9969(01)00098-X

M3 - Article

C2 - 11743927

SN - 0003-9969

VL - 47

SP - 1

EP - 9

JO - Archives of oral biology

JF - Archives of oral biology

IS - 1

ER -

Expression of a sodium bicarbonate cotransporter in human parotid salivary glands

Abstract

Keywords

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