Expression of endothelial and leukocyte adhesion molecules interacellular adhesion molecule-1, E-selectin, and vascular cell adhesion molecule-1 in the bronchial mucosa in steady-state and allergen-induced asthma

A M Bentley; S R Durham; D S Robinson; G Menz; C Storz; O Cromwell; A B Kay; A J Wardlaw

Expression of endothelial and leukocyte adhesion molecules interacellular adhesion molecule-1, E-selectin, and vascular cell adhesion molecule-1 in the bronchial mucosa in steady-state and allergen-induced asthma

A M Bentley, S R Durham, D S Robinson, G Menz, C Storz, O Cromwell, A B Kay, A J Wardlaw

Division of Immunology, Immunity to Infection and Respiratory Medicine

National Heart, Lung & Blood Institute (NHBLI)

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: Interactions between cell adhesion molecules and their ligands are an integral part of inflammatory processes and may have direct relevance to the pathology of asthma.

METHODS: Immunostaining with antibodies to cell adhesion molecules was performed on bronchial biopsy specimens from persons with intrinsic and extrinsic asthma, normal nonasthmatic control subjects, and patients with asthma after allergen challenge.

RESULTS: There was constitutive expression of intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1 (VCAM-1) in patients with intrinsic and extrinsic asthma and in control subjects. Compared with control subjects, ICAM-1 and E-selectin staining in the submucosa was greater in the intrinsic asthmatic group for intensity (p < 0.02, p < 0.05) and extent (p < 0.01, p < 0.05) of staining, respectively. No differences were observed between patients with extrinsic asthma and normal control subjects, and VCAM-1 expression did not differ among the groups. Epithelial expression of ICAM-1 was more frequent in patients with asthma compared with normal control subjects (p < 0.05). Compared with diluent challenge, bronchial biopsy specimens obtained 24 hours after allergen challenge revealed no significant differences in intensity or extent of staining for ICAM-1, E-selectin, or VCAM-1. After allergen challenge, the intensity and extent of both VCAM-1 and ICAM-1 expression correlated significantly with the number of eosinophils (cells positive for major basic protein). Epithelial ICAM-1 expression was more frequently observed after allergen challenge than after diluent challenge (p < 0.02).

CONCLUSIONS: The data suggest a complex pattern of regulation for ICAM-1, E-selectin, and VCAM-1 in vivo, where they may reflect the degree of ongoing inflammation in asthma.

Original language	English
Pages (from-to)	857-68
Number of pages	12
Journal	The Journal of allergy and clinical immunology
Volume	92
Issue number	6
Publication status	Published - Dec 1993

Keywords

Adult
Allergens/administration & dosage
Asthma/etiology
Bronchi/immunology
Cell Adhesion Molecules/metabolism
E-Selectin
Female
Forced Expiratory Volume
Humans
Immunohistochemistry
Intercellular Adhesion Molecule-1
Male
Middle Aged
Mucous Membrane/immunology
Vascular Cell Adhesion Molecule-1

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Bentley, A. M., Durham, S. R., Robinson, D. S., Menz, G., Storz, C., Cromwell, O., Kay, A. B., & Wardlaw, A. J. (1993). Expression of endothelial and leukocyte adhesion molecules interacellular adhesion molecule-1, E-selectin, and vascular cell adhesion molecule-1 in the bronchial mucosa in steady-state and allergen-induced asthma. The Journal of allergy and clinical immunology, 92(6), 857-68.

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title = "Expression of endothelial and leukocyte adhesion molecules interacellular adhesion molecule-1, E-selectin, and vascular cell adhesion molecule-1 in the bronchial mucosa in steady-state and allergen-induced asthma",

abstract = "BACKGROUND: Interactions between cell adhesion molecules and their ligands are an integral part of inflammatory processes and may have direct relevance to the pathology of asthma.METHODS: Immunostaining with antibodies to cell adhesion molecules was performed on bronchial biopsy specimens from persons with intrinsic and extrinsic asthma, normal nonasthmatic control subjects, and patients with asthma after allergen challenge.RESULTS: There was constitutive expression of intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1 (VCAM-1) in patients with intrinsic and extrinsic asthma and in control subjects. Compared with control subjects, ICAM-1 and E-selectin staining in the submucosa was greater in the intrinsic asthmatic group for intensity (p < 0.02, p < 0.05) and extent (p < 0.01, p < 0.05) of staining, respectively. No differences were observed between patients with extrinsic asthma and normal control subjects, and VCAM-1 expression did not differ among the groups. Epithelial expression of ICAM-1 was more frequent in patients with asthma compared with normal control subjects (p < 0.05). Compared with diluent challenge, bronchial biopsy specimens obtained 24 hours after allergen challenge revealed no significant differences in intensity or extent of staining for ICAM-1, E-selectin, or VCAM-1. After allergen challenge, the intensity and extent of both VCAM-1 and ICAM-1 expression correlated significantly with the number of eosinophils (cells positive for major basic protein). Epithelial ICAM-1 expression was more frequently observed after allergen challenge than after diluent challenge (p < 0.02).CONCLUSIONS: The data suggest a complex pattern of regulation for ICAM-1, E-selectin, and VCAM-1 in vivo, where they may reflect the degree of ongoing inflammation in asthma.",

keywords = "Adult, Allergens/administration & dosage, Asthma/etiology, Bronchi/immunology, Cell Adhesion Molecules/metabolism, E-Selectin, Female, Forced Expiratory Volume, Humans, Immunohistochemistry, Intercellular Adhesion Molecule-1, Male, Middle Aged, Mucous Membrane/immunology, Vascular Cell Adhesion Molecule-1",

author = "Bentley, {A M} and Durham, {S R} and Robinson, {D S} and G Menz and C Storz and O Cromwell and Kay, {A B} and Wardlaw, {A J}",

year = "1993",

month = dec,

language = "English",

volume = "92",

pages = "857--68",

journal = "The Journal of allergy and clinical immunology",

issn = "0091-6749",

publisher = "Mosby, Incorporated",

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Bentley, AM, Durham, SR, Robinson, DS, Menz, G, Storz, C, Cromwell, O, Kay, AB & Wardlaw, AJ 1993, 'Expression of endothelial and leukocyte adhesion molecules interacellular adhesion molecule-1, E-selectin, and vascular cell adhesion molecule-1 in the bronchial mucosa in steady-state and allergen-induced asthma', The Journal of allergy and clinical immunology, vol. 92, no. 6, pp. 857-68.

Expression of endothelial and leukocyte adhesion molecules interacellular adhesion molecule-1, E-selectin, and vascular cell adhesion molecule-1 in the bronchial mucosa in steady-state and allergen-induced asthma. / Bentley, A M; Durham, S R; Robinson, D S et al.
In: The Journal of allergy and clinical immunology, Vol. 92, No. 6, 12.1993, p. 857-68.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Expression of endothelial and leukocyte adhesion molecules interacellular adhesion molecule-1, E-selectin, and vascular cell adhesion molecule-1 in the bronchial mucosa in steady-state and allergen-induced asthma

AU - Bentley, A M

AU - Durham, S R

AU - Robinson, D S

AU - Menz, G

AU - Storz, C

AU - Cromwell, O

AU - Kay, A B

AU - Wardlaw, A J

PY - 1993/12

Y1 - 1993/12

N2 - BACKGROUND: Interactions between cell adhesion molecules and their ligands are an integral part of inflammatory processes and may have direct relevance to the pathology of asthma.METHODS: Immunostaining with antibodies to cell adhesion molecules was performed on bronchial biopsy specimens from persons with intrinsic and extrinsic asthma, normal nonasthmatic control subjects, and patients with asthma after allergen challenge.RESULTS: There was constitutive expression of intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1 (VCAM-1) in patients with intrinsic and extrinsic asthma and in control subjects. Compared with control subjects, ICAM-1 and E-selectin staining in the submucosa was greater in the intrinsic asthmatic group for intensity (p < 0.02, p < 0.05) and extent (p < 0.01, p < 0.05) of staining, respectively. No differences were observed between patients with extrinsic asthma and normal control subjects, and VCAM-1 expression did not differ among the groups. Epithelial expression of ICAM-1 was more frequent in patients with asthma compared with normal control subjects (p < 0.05). Compared with diluent challenge, bronchial biopsy specimens obtained 24 hours after allergen challenge revealed no significant differences in intensity or extent of staining for ICAM-1, E-selectin, or VCAM-1. After allergen challenge, the intensity and extent of both VCAM-1 and ICAM-1 expression correlated significantly with the number of eosinophils (cells positive for major basic protein). Epithelial ICAM-1 expression was more frequently observed after allergen challenge than after diluent challenge (p < 0.02).CONCLUSIONS: The data suggest a complex pattern of regulation for ICAM-1, E-selectin, and VCAM-1 in vivo, where they may reflect the degree of ongoing inflammation in asthma.

AB - BACKGROUND: Interactions between cell adhesion molecules and their ligands are an integral part of inflammatory processes and may have direct relevance to the pathology of asthma.METHODS: Immunostaining with antibodies to cell adhesion molecules was performed on bronchial biopsy specimens from persons with intrinsic and extrinsic asthma, normal nonasthmatic control subjects, and patients with asthma after allergen challenge.RESULTS: There was constitutive expression of intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1 (VCAM-1) in patients with intrinsic and extrinsic asthma and in control subjects. Compared with control subjects, ICAM-1 and E-selectin staining in the submucosa was greater in the intrinsic asthmatic group for intensity (p < 0.02, p < 0.05) and extent (p < 0.01, p < 0.05) of staining, respectively. No differences were observed between patients with extrinsic asthma and normal control subjects, and VCAM-1 expression did not differ among the groups. Epithelial expression of ICAM-1 was more frequent in patients with asthma compared with normal control subjects (p < 0.05). Compared with diluent challenge, bronchial biopsy specimens obtained 24 hours after allergen challenge revealed no significant differences in intensity or extent of staining for ICAM-1, E-selectin, or VCAM-1. After allergen challenge, the intensity and extent of both VCAM-1 and ICAM-1 expression correlated significantly with the number of eosinophils (cells positive for major basic protein). Epithelial ICAM-1 expression was more frequently observed after allergen challenge than after diluent challenge (p < 0.02).CONCLUSIONS: The data suggest a complex pattern of regulation for ICAM-1, E-selectin, and VCAM-1 in vivo, where they may reflect the degree of ongoing inflammation in asthma.

KW - Adult

KW - Allergens/administration & dosage

KW - Asthma/etiology

KW - Bronchi/immunology

KW - Cell Adhesion Molecules/metabolism

KW - E-Selectin

KW - Female

KW - Forced Expiratory Volume

KW - Humans

KW - Immunohistochemistry

KW - Intercellular Adhesion Molecule-1

KW - Male

KW - Middle Aged

KW - Mucous Membrane/immunology

KW - Vascular Cell Adhesion Molecule-1

M3 - Article

C2 - 7505008

SN - 0091-6749

VL - 92

SP - 857

EP - 868

JO - The Journal of allergy and clinical immunology

JF - The Journal of allergy and clinical immunology

IS - 6

ER -