Expression of glucose transporters GLUT-1, GLUT-3, GLUT-9 and HIF-1α in normal and degenerate human intervertebral disc

S. M. Richardson, R. Knowles, J. Tyler, A. Mobasheri, J. A. Hoyland

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    Abstract

    The glucose transporters GLUT-1 and GLUT-3 are targets of the hypoxia-inducible transcription factor HIF-1α and it has been shown that nucleus pulposus (NP) cells in rat intervertebral discs (IVD) express both HIF-1α and GLUT-1. However, there is limited data on the expression of HIF-1α and GLUTs in human IVD. The aim here was to (1) determine whether, like articular chondrocytes, human IVD cells express GLUT-1, 3 and 9 and whether there was any co-expression with HIF-1α; and (2) to localise expression of the GLUT isoforms in the disc and identify any changes during degeneration. Real-time PCR was used to identify expression of GLUT1, 3, 9 and HIF-1α mRNAs and immunohistochemistry was used to analyse protein expression and localisation of GLUTs in normal and degenerate IVD biopsies. Results confirmed HIF-1α, GLUT1, 3 and 9 mRNA expression in NP and AF and co-expression of each GLUT isoform with HIF-1α in the NP, but not the AF. Immunohistochemistry demonstrated regional differences in GLUT expression, with the highest expression being in the NP. GLUT expression also changed as degeneration progressed. This study demonstrates that NP and AF cells have different GLUT expression profiles that suggest regional differences in the metabolic nature of the human IVD and that this environment changes during degeneration. © 2007 Springer-Verlag.
    Original languageEnglish
    Pages (from-to)503-511
    Number of pages8
    JournalHistochemistry and cell biology
    Volume129
    Issue number4
    DOIs
    Publication statusPublished - Apr 2008

    Keywords

    • Degeneration
    • GLUT-1
    • GLUT-3
    • GLUT-9
    • HIF-1α
    • Hypoxia
    • Immunohistochemistry
    • Intervertebral disc
    • Real-time PCR

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