Expression of nitric oxide synthase isoforms and arginase in normal human skin and chronic venous leg ulcers

Seham A. Abd-El-Aleem, Mark W J Ferguson, Ian Appleton, Sadeo Kairsingh, Edward B. Jude, Kelly Jones, Charles N. McCollum, Grenham W. Ireland

    Research output: Contribution to journalArticlepeer-review


    Chronic venous ulcers, an example of abnormal wound healing, show chronic inflammation with defective matrix deposition which together with the underlying vascular pathology, result in delayed healing. L-arginine is known to be metabolized by one of two pathways: nitric oxide synthase (NOS), producing nitric oxide (NO), or arginase, producing ornitlune. NO is involved in many pathological conditions including vascular and inflammatory disorders. This study therefore investigated the distribution, level and activity of NOS and arginase in chronic venous ulcers in comparison with normal skin, using immunocytochemistry, western blotting, and enzyme assays. The results demonstrated an increased distribution of both NOS and arginase in chronic venous ulcer tissue compared with normal skin, with inflammatory cells and vascular endothelial cells as the main sources. These data were confirmed by western blot analysis, which showed increased levels of both enzymes in chronic venous ulcers. Moreover, there was significantly increased activity of both total NOS (p
    Original languageEnglish
    Pages (from-to)434-442
    Number of pages8
    JournalJournal of Pathology
    Issue number4
    Publication statusPublished - 2000


    • Arginase
    • Chronic venous ulcer
    • Endothelial nitric oxide synthase
    • Inducible nitric oxide synthase
    • Nitric oxide
    • Nitric oxide synthase


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